Use of a novel microfluidic disk in the analysis of single-cell viability and the application to Jurkat cells

Autor:	Kenji Matoba, Shunsuke Furutani, Izumi Kubo
Rok vydání:	2010
Předmět:	Microchannel Cell Survival Microfluidics Cell Bioengineering Cell Separation Biology Microfluidic Analytical Techniques Applied Microbiology and Biotechnology Jurkat cells Cell biology Jurkat Cells medicine.anatomical_structure Cell separation medicine Humans Viability assay Biotechnology
Zdroj:	Journal of bioscience and bioengineering. 112(1)
ISSN:	1347-4421
Popis:	Jurkat cells were trapped in the microchambers of a novel disk-shaped cell separation device and stained with Cellstain. Approximately 90% of the cells were living. Single cells were isolated with a branching microchannel after rotation at 4500 rpm for 30 s, demonstrating that a living single cell could be trapped in the microchambers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c2eace01e564dd9a3aaa3823879fa95 https://pubmed.ncbi.nlm.nih.gov/21497547 Zobrazit plný text záznamu Full Text from ScienceDirect