Antibody-based surface plasmon resonance detection of intact viral pathogen
| Autor: | Sung June Kim, Donghyun Kim, Jennifer Lee, Michael L. Shuler, József P. Hajós, Hyoungwon Baac |
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| Rok vydání: | 2006 |
| Předmět: |
biology
viruses fungi Nuclear Polyhedrosis Virus Bioengineering Tobamovirus Biosensing Techniques Surface Plasmon Resonance Antibodies Viral biology.organism_classification Applied Microbiology and Biotechnology Virology Virus Autographa californica Viruses Tobacco mosaic virus biology.protein Surface plasmon resonance Protein A Pathogen Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 94:815-819 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.20882 |
| Popis: | Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors. |
| Databáze: | OpenAIRE |
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