Cardiolipin: a stereospecifically spin-labeled analogue and its specific enzymic hydrolysis
| Autor: | John Jacobus, Gary L. Powell, Michael B. Cable |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Multidisciplinary
Phospholipase C biology Cardiolipins Phospholipase D Stereochemistry Monolysocardiolipin Molecular Conformation Phospholipid Context (language use) Phospholipase Substrate Specificity chemistry.chemical_compound Phospholipase A2 chemistry Biochemistry Phospholipases Cardiolipin biology.protein Spin Labels lipids (amino acids peptides and proteins) Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 75:1227-1231 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.75.3.1227 |
| Popis: | The spin-labeled cardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-(16-doxylstearoyl)glycero(3)phosphol]-sn-glycerol has been prepared. The stereoselective synthesis makes use of the monolysocardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-lyso-sn-glycero(3)phospho]-sn-glycerol, available from the stereospecific hydrolysis of cardiolipin by phospholipase A2 (phosphatide 2-acylhydrolase, EC 3.1.1.4) of Trimeresurus flavoviridis. The results of treatment of the spin-labeled cardiolipin with the cardiolipin-specific phospholipase D (phosphatidylcholine phosphatidohydrolase, EC 3.1.4.4) (Hemophilus parainfluenzae) of known specificity and with phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) of Bacillus cereus are consistent with the assigned structure. The spin-labeled cardiolipin is further characterized and the unique features of this diastereomer are discussed in the context of the unusual stereochemistry of the natural phospholipid. |
| Databáze: | OpenAIRE |
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