Effect of modulation of the transferrin receptor on gallium-67 uptake and cytotoxicity in lymphoma cell lines
| Autor: | Angelika M. Dräger, A.E. van Leeuwen-Stok, G. J. J. Teule, Gerrit Jan Schuurhuis, A. W. J. Visser-Platier, Peter C. Huijgens |
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| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
medicine.medical_specialty Lymphoma Cell Survival Cell Gene Expression Antineoplastic Agents Gallium Radioisotopes Transferrin receptor Biology Cell Line Cell cycle phase Cell surface receptor Internal medicine Receptors Transferrin Tumor Cells Cultured medicine Humans Hydroxyurea Clonogenic assay Cytotoxicity Tumor Stem Cell Assay chemistry.chemical_classification Cell Cycle Cytarabine Biological Transport Cell cycle Flow Cytometry Methotrexate medicine.anatomical_structure Endocrinology Oncology chemistry Transferrin Cancer research Cell Division Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Gallium-67 is a radionuclide that accumulates in haematological malignancies and is used for diagnostic purposes. Uptake of 67Ga into the cell occurs via the transferrin receptor, which is differentially expressed during the various cell cycle phases. With the aim of selectively increasing 67Ga uptake, we studied whether the transferrin receptor (TfR) expression could be modulated in the U937 and U715 lymphoma cell lines by cytostatic drugs inducing cell cycle phase accumulation. We tested clinically relevant drugs such as 1-beta-D-arabinofuranosylcytosine (Ara-C), hydroxyurea and methotrexate. Cytotoxicity was determined by testing the clonogenic capacity of the lymphoma cell lines. All three drugs induced an increase in S-phase content, TfR expression and 67Ga uptake in U937 and U715 single cells. The combinations of drugs and 67Ga resulted in an additive effect on the clonogenic capacity. In U937 spheroids, cultured by the fibrin clot technique, we found an accumulation in the S-phase too as well as an increase of the transferrin receptor expression after Ara-C preincubation. As in single cells 67Ga uptake was increased without synergistic effects on the clonogenic capacity. In conclusion, priming with drugs induces increased transferrin receptor expression and 67Ga uptake. Inhibition of clonogenic capacity was additive rather than synergistic. |
| Databáze: | OpenAIRE |
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