Resistance mechanisms and genetic relatedness among carbapenem-resistant Pseudomonas aeruginosa isolates from three major hospitals in Hanoi, Vietnam (2011–15)
| Autor: | Van Anh Tran, Duy Thai Pham, H. Rogier van Doorn, Hai Anh Tran, Ha My Pham, Nhu Duong Tran, Lay-Myint Yoshida, Thi Ngoc Bich Vu, Khanh Linh Trinh, Masato Suzuki, Hong Son Trinh, Son Tung Trinh, Anne-Laure Bañuls, Keigo Shibayama, Duc Anh Dang, Viet Thanh Le, Dieu Linh Tran, Phuong Thom Vu, Thi Hong Hanh Ngo, Huy Hoang Tran, Thi Vu Nga Luu, Minh Thao Nguyen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Imipenem Phylogenetic tree Pseudomonas aeruginosa 030106 microbiology Biology medicine.disease_cause biology.organism_classification Microbiology Sequence-tagged site 03 medical and health sciences 030104 developmental biology medicine Carbapenem resistant Pseudomonas aeruginosa Multilocus sequence typing Gene Bacteria medicine.drug |
| Zdroj: | JAC-Antimicrobial Resistance. 3 |
| ISSN: | 2632-1823 |
| Popis: | Background MDR bacteria including carbapenem-resistant Pseudomonas aeruginosa are recognized as an important cause of hospital-acquired infections worldwide. This investigation seeks to determine the molecular characterization and antibiotic resistance genes associated with carbapenem-resistant P. aeruginosa. Methods We conducted WGS and phylogenetic analysis of 72 carbapenem-resistant P. aeruginosa isolated from hospital-acquired infection patients from August 2011 to March 2015 in three major hospitals in Hanoi, Vietnam. Results We identified three variants of IMP gene, among which blaIMP-15 was the most frequent (n = 34) in comparison to blaIMP-26 (n = 2) and blaIMP-51 (n = 12). We observed two isolates with imipenem MIC >128 mg/L that co-harboured blaIMP-15 and blaDIM-1 genes and seven isolates (imipenem MIC > 128 mg/L) with a blaKPC-1 gene from the same hospital. MLST data shows that these 72 isolates belong to 18 STs and phylogenetic tree analysis has divided these isolates into nine groups. Conclusions Our results provide evidence that not only blaIMP-26 but other IMP variants such as blaIMP-15 and blaIMP-51 genes and several STs (ST235, ST244, ST277, ST310, ST773 and ST3151) have been disseminating in healthcare settings in Vietnam. In addition, we report the emergence of two isolates belonging to ST1240 and ST3340 that harboured two important carbapenemase genes (blaIMP-15 and blaDIM-1) and seven isolates belonging to ST3151 of P. aeruginosa that carried the blaKPC-1 gene in Vietnam, which could potentially cause serious restricted availability of treatment options in healthcare settings. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|