Phenanthrene contamination and ploidy level affect the rhizosphere bacterial communities of Spartina spp
| Autor: | Cécile Monard, Armand Cavé-Radet, Abdelhak El Amrani, Armel Salmon, Sara Correa-García, Etienne Yergeau, Malika L. Aïnouche |
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| Přispěvatelé: | Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), AC-R was supported by an Erasmus+ mobility scholarship from the European Commission. This work was supported by the Ministère de l'Enseignement Supérieur et de la Recherche, the CNRS (Centre National de la Recherche Scientifique), the Observatoire des Sciences et de l'Univers de Rennes (OSUR) and a NSERC (Natural Science and Engineering Research Council of Canada) Discovery Grant (2014-05274 to EY). This research was enabled in part by support provided by Calcul Québec (www.calculquebec.ca) and Compute Canada (www.computecanada.ca)., Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Scientifique [Québec] (INRS), A.C.-R. was supported by an Erasmus+ mobility scholarship from the European Commission. This work was supported by the Ministère de l’Enseignement Supérieur et de la Recherche, by the CNRS, the Observatoire des Sciences et de l’Univers de Rennes (OSUR) and by a NSERC Discovery Grant (2014-05274 to EY). This research was enabled in part by supportprovided by Calcul Québec (www.calculquebec.ca) and Compute Canada (www.computecanada.ca)., Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
010501 environmental sciences Biology 01 natural sciences Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences rhizoremediation RNA Ribosomal 16S Phenanthrene Botany Genotype Soil Pollutants Polycyclic Aromatic Hydrocarbons Soil Microbiology polyploidy 0105 earth and related environmental sciences Rhizosphere geography Spartina geography.geographical_feature_category Ploidies Ecology Interspecific competition Phenanthrenes biology.organism_classification bacterial communities Phytoremediation 030104 developmental biology Biodegradation Environmental Salt marsh Ploidy [SDE.BE]Environmental Sciences/Biodiversity and Ecology rhizosphere Bacteria |
| Zdroj: | FEMS Microbiology Ecology FEMS Microbiology Ecology, Wiley-Blackwell, 2020, 96 (10), ⟨10.1093/femsec/fiaa156⟩ FEMS Microbiology Ecology, 2020, 96 (10), pp.AA156. ⟨10.1093/femsec/fiaa156⟩ |
| ISSN: | 0168-6496 1574-6941 |
| Popis: | Spartina spp. are widely distributed salt marsh plants that have a recent history of hybridization and polyploidization. These events have resulted in a heightened tolerance to hydrocarbon contaminants, but the effects of this phenomenon on the rhizosphere microbial communities are unknown. Here, we grew two parental Spartina species, their hybrid and the resulting allopolyploid in salt marsh sediments that were contaminated or not with phenanthrene. The DNA from the rhizosphere soil was extracted and the bacterial 16S rRNA gene was amplified and sequenced, whereas the abundances of the genes encoding for the PAH (polycyclic aromatic hydrocarbon) ring-hydroxylating dioxygenase (RHD) of Gram-negative and Gram-positive bacteria were quantified by real-time PCR. Both the contamination and the plant genotype significantly affected the bacterial communities. In particular, the allopolyploid S. anglica harbored a more diverse bacterial community in its rhizosphere. The interspecific hybrid and the allopolyploid also harbored significantly more copies of the PAH-RHD gene of Gram-negative bacteria in their rhizosphere than the parental species, irrespective of the contamination treatments. Overall, our results are showing that the recent polyploidization events in the Spartina affected its rhizosphere bacterial communities, both under normal and contaminated conditions, possibly increasing its phytoremediation potential. |
| Databáze: | OpenAIRE |
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