Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome
| Autor: | Li Gao, Chao Gao, Yuan Zhang, Mei Li, Dai Xiao-nan, Diao Fei-yang, Gao Lingling, Xiang Ma, Cui Yugui, Xu Boqun, Dai Xue, Liu Jia-yin |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
lcsh:RC648-665 Article Subject Microarray medicine.diagnostic_test endocrine system diseases Endocrine and Autonomic Systems Endocrinology Diabetes and Metabolism Hyperandrogenism Biology medicine.disease Polycystic ovary lcsh:Diseases of the endocrine glands. Clinical endocrinology Pathophysiology female genital diseases and pregnancy complications Endocrinology Western blot Theca Internal medicine medicine Immunohistochemistry Cellular localization Research Article |
| Zdroj: | International Journal of Endocrinology International Journal of Endocrinology, Vol 2013 (2013) |
| ISSN: | 1687-8337 |
| Popis: | Background. We previously found that expression of SET gene was up-regulated in polycystic ovaries by using microarray. It suggested that SET may be an attractive candidate regulator involved in the pathophysiology of polycystic ovary syndrome (PCOS). In this study, expression and cellular localization of SET protein were investigated in human polycystic and normal ovaries.Method. Ovarian tissues, six normal ovaries and six polycystic ovaries, were collected during transsexual operation and surgical treatment with the signed consent form. The cellular localization of SET protein was observed by immunohistochemistry. The expression levels of SET protein were analyzed by Western Blot.Result. SET protein was expressed predominantly in the theca cells and oocytes of human ovarian follicles in both PCOS ovarian tissues and normal ovarian tissues. The level of SET protein expression in polycystic ovaries was triple higher than that in normal ovaries(P<0.05).Conclusion. SET was overexpressed in polycystic ovaries more than that in normal ovaries. Combined with its localization in theca cells, SET may participate in regulating ovarian androgen biosynthesis and the pathophysiology of hyperandrogenism in PCOS. |
| Databáze: | OpenAIRE |
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