Is aromatic terpenoid composition of grapes in Northwestern Iberian wine cultivars related to variation in VviDXS1 gene?
| Autor: | José M. Martínez-Zapater, Mar Vilanova, Javier Ibáñez, Rafael Torres-Pérez, Luisa Freire, Carolina Royo, Pablo Carbonell-Bejerano |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Xunta de Galicia |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Aroma of wine Soil Science Plant Science Horticulture Biology 01 natural sciences Biochemistry 03 medical and health sciences Cultivar Gene Aroma Wine fungi food and beverages biology.organism_classification Terpenoid Haplotype diversity Muscat aroma White (mutation) 030104 developmental biology DXS Monoterpenes Composition (visual arts) C13-norisoprenoids Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1878-5093 |
| Popis: | BACKGROUND: Monoterpenes and C13-norisoprenoids are key terpenoid compounds for wine aroma. The enzyme encoded by VviDXS1 participates in terpenoid biosynthesis in grapevine fruits and gain-of-function mutations in this gene lead to characteristic muscat aroma. OBJECTIVE: To assess for VviDXS1 contribution to aroma variation in Northwestern Iberian wine cultivars, we resequenced this gene in 111 cultivars and compared grape juice terpenic composition in 12 of them. METHODS: VviDXS1 was capture-targeted for resequencing with Illumina paired-end reads, SAMtools was used for variant calling and gene haplotypes were reconstructed with PHASE. Monoterpenes and C13-norisoprenoids were quantified in free and glycosidically-bound forms from grape juice by gas chromatography-mass spectrometry. RESULTS: Terpenic composition discriminated between muscat, terpenic and neutral profiles across cultivars. While the terpenic profile of Loureira and Albariño white cultivars was not associated with muscat-like mutations, Albariño carries a V34L substitution in VviDXS1 that is also present in other aromatic cultivars and was not reported before. Tempranillo and Cabernet Sauvignon red cultivars accumulated higher levels of C13-norisoprenoids, which was not associated with specific variation in VviDXS1. CONCLUSIONS: Apart from the uncharacterized substitution present in Albariño, findings suggest that terpenoid pathway-related genes other than VviDXS1 could contribute to the aromatic attributes of these cultivars. This work was partially supported by project RTA 2011-00100-C05-00, BIO2011-26229 and BIO2017-86375 by the Spanish MICINN and IN841C by the Xunta de Galicia. |
| Databáze: | OpenAIRE |
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