The hyaluronan-mediated motility receptor RHAMM promotes growth, invasiveness and dissemination of colorectal cancer
| Autor: | Luigi Terracciano, Giandomenica Iezzi, Inti Zlobec, Valentina Mele, Irene Keller, Viktor Kölzer, Dennis Pfaff, Irene Centeno, Alessandro Lugli, Zahnd Stefan, Heather Dawson, Manuele G. Muraro, Lena Sokol |
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| Přispěvatelé: | University of Zurich, Lugli, Alessandro |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Colorectal cancer proliferation colorectal cancer 610 Medicine & health Metastasis Flow cytometry RHAMM 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation 10049 Institute of Pathology and Molecular Pathology metastasis Medicine medicine.diagnostic_test business.industry Cell growth Cell migration Cell cycle invasion medicine.disease Hyaluronan-mediated motility receptor digestive system diseases 3. Good health 030104 developmental biology Oncology 030220 oncology & carcinogenesis Immunology Cancer research 570 Life sciences biology 2730 Oncology business Research Paper |
| Zdroj: | Mele, Valentina; Sokol, Lena; Kölzer, Viktor; Pfaff, Dennis; Muraro, Manuele Giuseppe; Keller, Irene; Zahnd, Stefan Patrick; Centeno Ramos, Irene; Terracciano, Luigi Maria; Dawson, Heather; Zlobec, Inti; Iezzi, Giandomenica; Lugli, Alessandro (2017). The hyaluronan-mediated motility receptor RHAMM promotes growth, invasiveness and dissemination of colorectal cancer. OncoTarget, 8(41), pp. 70617-70629. Impact Journals LLC 10.18632/oncotarget.19904 Oncotarget |
| DOI: | 10.5167/uzh-174596 |
| Popis: | In colorectal cancer (CRC), RHAMM is an independent adverse prognostic factor. The aim of the study was therefore to investigate on the role of RHAMM as a potential direct driver of cell proliferation and migration in CRC cell lines and to identify pathways dependent on RHAMM in human CRC. Proliferation, cell cycle alterations and invasive capacity were tested in two RHAMM- and control- knockdown CRC cell lines by flow cytometry and in vitro assays. Tumorigenicity and metastasis formation was assessed in immunodeficient mice. RNA-Seq and immunohistochemistry was performed on six RHAMM+/- primary CRC tumors. In vitro, silencing of RHAMM inhibited CRC cell migration and invasion by 50% (p |
| Databáze: | OpenAIRE |
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