A COMPARISON OF SOME SIMPLIFIED LACTOBIONATE PRESERVATION SOLUTIONS WITH STANDARD UW SOLUTION AND EUROCOLLINS SOLUTION FOR PANCREAS PRESERVATION
| Autor: | Neville V. Jamieson, Yasuhiko Fukuda, Kiyohiko Dohi, Takashi Urushihara, Kazuo Sumimoto, M. Ikeda, Ryo Sumimoto, Hisao Ito |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Adenosine Allopurinol Sodium Organ Preservation Solutions Urology Blood sugar chemistry.chemical_element Hydroxyethyl starch Disaccharides chemistry.chemical_compound Raffinose medicine Animals Insulin Viaspan Transplantation Glucose tolerance test medicine.diagnostic_test business.industry Organ Preservation Glutathione Rats Surgery Solutions medicine.anatomical_structure Eurocollins solution chemistry Rats Inbred Lew Pancreas Transplantation Pancreas business medicine.drug |
| Zdroj: | Transplantation. 53:750-754 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-199204000-00008 |
| Popis: | Fifty-two rat pancreas transplants were performed to investigate which components of the UW solution were essential for successful pancreas preservation. LEW rats were used and the pancreata stored at 4 degrees C for 48 hr after flushing with commercial UW solution (ViaSpan, DuPont Pharmaceuticals) or a number of simplified solutions. Following storage the pancreata were transplanted into syngeneic recipient animals with streptozotocin-induced diabetes mellitus. Graft function was assessed by regular postoperative blood sugar measurements and a glucose tolerance test on the 14th postoperative day. With commercial UW solution, 4 of 9 recipients (44%) showed satisfactory graft function, while only one of 5 pancreata preserved using Eurocollins solution demonstrated satisfactory function. With solution A, in which hydroxyethyl starch and insulin were omitted from the standard UW solution, 3 of 7 recipients (43%) showed satisfactory function. Omission of glutathione, allopurinol, and adenosine from this solution (solution B) gave satisfactory function in 4 of 8 cases (50%). Substitution of raffinose in solution B with an equimolar concentration of glucose (solution C) resulted in acceptable function in 5 of 8 cases (62%). Increasing the raffinose concentration in solution B to 100 mM/L resulted in only 2 of 8 grafts (25%) with adequate function. By contrast, reversing the Na/K concentrations in solution A resulted in 100% (7/7) satisfactory graft function. We conclude that the rat pancreas can be successfully transplanted following 48-hr cold preservation using UW solution and some simplified versions, and that a substantially simplified lactobionate-based solution with a reversed sodium/potassium ratio improved survival. |
| Databáze: | OpenAIRE |
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