Granulocyte-Colony Stimulating Factor Augments Neovascularization Induced by Bone Marrow Transplantation in Rat Hindlimb Ischemia
| Autor: | Kaname Akioka, Daisuke Nishiya, Takashi Omura, Kazuhide Takeuchi, Hiroshi Iwao, Yasuhiro Takagi, Minoru Yoshiyama, Takanori Kusuyama, Junichi Yoshikawa, Soichiro Enomoto, Ryo Matsumoto |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Pathology Time Factors Angiogenesis Neovascularization Physiologic Hindlimb Endothelial progenitor cell Neovascularization chemistry.chemical_compound Ischemia Granulocyte Colony-Stimulating Factor medicine Laser-Doppler Flowmetry Animals RNA Messenger Progenitor cell Muscle Skeletal Bone Marrow Transplantation Cell Proliferation Pharmacology business.industry lcsh:RM1-950 Angiography Alkaline Phosphatase Granulocyte colony-stimulating factor Surgery Rats Vascular endothelial growth factor medicine.anatomical_structure lcsh:Therapeutics. Pharmacology chemistry Rats Inbred Lew Regional Blood Flow Molecular Medicine Angiogenesis Inducing Agents Fibroblast Growth Factor 2 Bone marrow medicine.symptom business |
| Zdroj: | Journal of Pharmacological Sciences, Vol 99, Iss 1, Pp 45-51 (2005) |
| ISSN: | 1347-8613 |
| Popis: | Because granulocyte-colony stimulating factor (G-CSF) mobilizes bone marrow cells including endothelial progenitor cells, we examined whether G-CSF augments angiogenesis and collateral vessel formation induced by bone marrow-mononuclear cells transplantation (BMT). Unilateral hindlimb ischemia was surgically induced in Lewis rats. One week after surgery, administration of 100 mg/kg per day G-CSF significantly increased the laser Doppler blood perfusion index (LDBPI), number of angiographically detectable collateral vessels (angiographic score), and capillary density determined by alkaline phosphatase staining. In the BMT group (1 × 107 cells/rat) and the group with combined G-CSF treatment and BMT, LDBPI was significantly increased compared with that in the vehicle-treated group. In the BMT group, neovascularization was significantly increased as evidenced by the angiographic score and capillary density compared with the vehicle-treated group. Furthermore, the combination of G-CSF treatment and BMT augmented neovascularization compared with BMT alone, as evidenced by the angiographic score and capillary density. Moreover, G-CSF significantly increased vascular endothelial growth factor mRNA and fibroblast growth factor-2 mRNA in hindlimb muscle. In conclusion, G-CSF was found to augment neovascularization in rat hindlimb ischemia. Combined use of G-CSF treatment and BMT may be a useful strategy for therapeutic neovascularization in ischemic tissues. Keywords:: granulocyte-colony stimulating factor, bone marrow-mononuclear cell transplantation, angiogenesis, hindlimb ischemia, endothelial progenitor cell |
| Databáze: | OpenAIRE |
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