| Autor: |
Steven B, Symington, Abigail C, Toltin, Edwin, Murenzi, David, Lansky, John M, Clark |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Pesticide Biochemistry and Physiology. 189:105296 |
| ISSN: |
0048-3575 |
| Popis: |
Microtransplantation of neurolemma tissue fragments from mammalian brain into the plasma membrane of Xenopus laevis oocytes is a tool to examine the endogenous structure and function of various ion channels and receptors associated with the central nervous system. Microtransplanted neurolemma can originate from a variety of sources, contain ion channels and receptors in their native configuration, and are applicable to examine diseases associated with different channelopathies. Here, we examined potential age-related differences in voltage-sensitive sodium channel (VSSC) expression and concentration-dependent responses to pyrethroids following the microtransplantation of juvenile or adult rat brain tissue (neurolemma) into X. laevis oocytes. Using automated western blotting, adult neurolemma exhibited a 2.5-fold higher level of expression of VSSCs compared with juvenile neurolemma. The predominant isoform expressed in both tissues was Na |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect