AtGRP5, a vacuole-located glycine-rich protein involved in cell elongation
| Autor: | Gilbert Engler, Vanessa Cardeal, Amanda Mangeon, Rogério Margis, Vinicius Costa Galvão, Gilberto Sachetto-Martins, Adriana Dias Menezes-Salgueiro, Cristina de Oliveira, Claudia Magioli |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Signal peptide Transgene Arabidopsis Glycine Plant Science Green fluorescent protein Gene Expression Regulation Plant RNA interference Sense (molecular biology) Genetics Arabidopsis thaliana Cell Shape Microscopy Confocal biology Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction fungi Protein primary structure food and beverages Plants Genetically Modified biology.organism_classification Cell biology Vacuoles |
| Zdroj: | Planta. 230:253-265 |
| ISSN: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-009-0940-4 |
| Popis: | Although several glycine-rich protein (GRP) genes were isolated and characterized, very little is known about their function. The primary structure of AtGRP5 from Arabidopsis thaliana has a signal peptide followed by a region with high glycine content. In this work, green fluorescent protein fusions were obtained in order to characterize the sub-cellular localization of the AtGRP5 protein. The results indicated that this protein is the first described vacuolar GRP. Sense, antisense and RNAi transgenic A. thaliana plants were generated and analyzed phenotypically. Plants overexpressing AtGRP5 showed longer roots and an enhanced elongation of the inflorescence axis, while antisense and RNAi plants demonstrated the opposite phenotype. The analysis of a knockout T-DNA line corroborates the phenotypes obtained with the antisense and RNAi plants. Altogether, these results suggest that this vacuolar GRP could be involved in organ growth by promoting cell elongation processes. |
| Databáze: | OpenAIRE |
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