Effects of Sesquiterpenes Isolated From Largehead Atractylodes Rhizome on Growth, Migration, and Differentiation of B16 Melanoma Cells
| Autor: | Wang-Fun Fong, Hui Wang, Zhi-Ling Yu, Gui-Xin Chou, Jian-Hong Chu, Yan Ye |
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| Rok vydání: | 2010 |
| Předmět: |
MAPK/ERK pathway
Lactams Cell Survival Cellular differentiation Melanoma Experimental Cell Growth Processes Sesquiterpene Lactones Mice chemistry.chemical_compound Cell Movement Tumor Cells Cultured Animals Extracellular Signal-Regulated MAP Kinases Protein kinase B PI3K/AKT/mTOR pathway biology Plant Extracts Cell Differentiation Atractylodes biology.organism_classification Oncogene Protein v-akt Complementary and alternative medicine Oncology Biochemistry chemistry Cancer cell Plant Preparations Signal transduction Sesquiterpenes Rhizome Signal Transduction |
| Zdroj: | Integrative Cancer Therapies. 10:92-100 |
| ISSN: | 1552-695X 1534-7354 |
| DOI: | 10.1177/1534735410378660 |
| Popis: | The aims of this study were to isolate sesquiterpene compounds from the largehead atractylodes rhizome (LAR) and to investigate their effects on B16 cancer cells. A total of 8 sesquiterpenes from LAR were identified, of which eudesm-4 (15), 7-diene-9α, 11-diol (7) was isolated for the first time. All 8 compounds inhibited growth of B16 cells, and atractylenolide I (AT-I), atractylenolide II (AT-II), and atractylenolactam (ATR) were the most potent, with IC50 values of 76.46, 84.02, and 54.88 μΜ, respectively. Monomer lactone or lactam structures in the 8 compounds appeared to be critical for their antiproliferative activities. In addition, AT-I, AT-II, and ATR could induce cell differentiation and inhibit cell migration. Western blot analysis indicated that 2 of the compounds, AT-I and AT-II, could inactivate ERK, where all 3 inhibited AKT activation, suggesting that Ras/ERK and PI3K/AKT signaling pathways are involved in the action mechanisms of the LAR sesquiterpene compounds. |
| Databáze: | OpenAIRE |
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