selectin and SDF-1 enhance the migration of mouse and human cardiac mesoangioblasts
Autor: | A Soldo, Diego Covarello, Roberto Latini, Maria Garcia Fernandez, Aurora Bernal, Fabiola Molla, Beatriz G. Gálvez, Giulio Cossu, N San Martín |
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Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
Male
medicine.medical_specialty Time Factors Endothelium Caveolin 1 migration Cell therapy Mice In vivo Cell Movement Internal medicine medicine Animals Humans L-Selectin Molecular Biology Original Paper biology Myocardium Stem Cells CD44 Cell Biology cytokines Chemokine CXCL12 Matrix Metalloproteinases Mice Inbred C57BL medicine.anatomical_structure Hyaluronan Receptors Ventricle regeneration Immunology biology.protein Cardiology L-selectin Stem cell homing Homing (hematopoietic) |
Zdroj: | Cell Death and Differentiation |
ISSN: | 1476-5403 1350-9047 |
Popis: | Efficient delivery of stem cells to heart regions is still a major problem for cell therapy. Here, we report experiments aimed to improve migration of mouse and human cardiac mesoangioblasts to the damaged heart. Cardiac mesoangioblasts were induced to transmigrate through the endothelium by factors released by cardiomyocytes or cytokines, among which stromal-derived factor 1 (SDF-1) was the most potent. Cardiac mesoangioblasts were also delivered into the left ventricular (LV) chamber of mice after coronary artery ligation (CAL), and their in vivo homing to the damaged heart was found to be quite modest. Pretreatment of cardiac mesoangioblasts with SDF-1 or transient expression of L-selectin induced a two- to three-fold increase in their transmigration and homing to the damaged heart. Therefore, combined pretreatment with SDF-1 and L-selectin generated modified cardiac mesoangioblasts, 50% of which, after injection into the LV chamber of mice early after CAL, home directly to the damaged free wall of the heart. Finally, modified mouse cardiac mesoangioblasts, injected into the LV chamber regenerate a larger surface of the ventricle in long-term experiments in comparison with their control counterparts. This study defines the requirements for efficient homing of cardiac mesoangioblasts to the damaged heart and offers a new potent tool to optimize efficiency of future cell therapy protocols for cardiovascular diseases. |
Databáze: | OpenAIRE |
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