| Autor: |
Zhilin Zhang, Rashmi Rekha Hazarika, Robert Schmitz, YINWEN ZHANG, Frank Johannes, Michele Serra |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Nat Plants |
| ISSN: |
2055-0278 |
| DOI: |
10.1038/s41477-021-01086-7 |
| Popis: |
Mistakes in the maintenance of CG methylation are a source of heritable epimutations in plants. Multigenerational surveys indicate that the rate of these stochastic events varies substantially across the genome, with some regions harbouring localized 'epimutation hotspots'. Using Arabidopsis as a model, we show that epimutation hotspots are indexed by a specific set of chromatin states that map to subregions of gene body methylation genes. Although these regions comprise only ~12% of all CGs in the genome, they account for ~63% of all epimutation events per unit time. Molecular profiling revealed that these regions contain unique sequence features, harbour steady-state intermediate methylation levels and act as putative targets of antagonistic DNA methylation pathways. We further demonstrate that experimentally induced shifts in steady-state methylation in these hotspot regions are sufficient to significantly alter local epimutation intensities. Our work lays the foundation for dissecting the molecular mechanisms and evolutionary consequences of epimutation hotspots in plants. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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