Supplementary Figure S5 from Mutant Calreticulin Requires Both Its Mutant C-terminus and the Thrombopoietin Receptor for Oncogenic Transformation

Autor: Ann Mullally, Fatima Al-Shahrour, Rebekka K. Schneider, Julie-Aurore Losman, Zuzana Tothova, Elizabeth A. Morgan, Ofir Wolach, Silvia Giannini, Natalie Florescu, Fabian Peisker, Amy Ko, Emily A. Rosen, Javier Perales-Patón, Edwin Chen, Nouran S. Abdelfattah, Shannon Elf
Rok vydání: 2023
DOI: 10.1158/2159-8290.22531500
Popis: Supplementary Figure S5. Immunoblotting of Calr-immunoprecipitated proteins and whole cell lysates from Ba/F3-Cas9 and Ba/F3-MPL-Cas9 cells targeted with Calr-directed CRISPR gene editing using a control guide, Calr guide m1, or Calr guide m2 demonstrates increased binding between MPL and mutant Calr (lane 3 - 4 (m1) and lane 5 -6 (m2)) as compared to wild type Calr (lane 2).
Databáze: OpenAIRE