Postexercise improvement in glucose uptake occurs concomitant with greater γ3-AMPK activation and AS160 phosphorylation in rat skeletal muscle
| Autor: | Haiyan Wang, Gregory D. Cartee, Laurie J. Goodyear, Edward B. Arias, Mark W. Pataky |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism Glucose uptake Deoxyglucose 03 medical and health sciences AMP-activated protein kinase Physical Conditioning Animal Physiology (medical) Internal medicine medicine Animals Phosphorylation Muscle Skeletal Protein kinase B biology Chemistry Adenylate Kinase Glucose transporter AMPK Skeletal muscle Rats Glucose 030104 developmental biology medicine.anatomical_structure Endocrinology Concomitant biology.protein Research Article |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 315:E859-E871 |
| ISSN: | 1522-1555 0193-1849 |
| Popis: | A single exercise session can increase insulin-stimulated glucose uptake (GU) by skeletal muscle, concomitant with greater Akt substrate of 160 kDa (AS160) phosphorylation on Akt-phosphosites (Thr642and Ser588) that regulate insulin-stimulated GU. Recent research using mouse skeletal muscle suggested that ex vivo 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) or electrically stimulated contractile activity-inducing increased γ3-AMPK activity and AS160 phosphorylation on a consensus AMPK-motif (Ser704) resulted in greater AS160 Thr642phosphorylation and GU by insulin-stimulated muscle. Our primary goal was to determine whether in vivo exercise that increases insulin-stimulated GU in rat skeletal muscle would also increase γ3-AMPK activity and AS160 site-selective phosphorylation (Ser588, Thr642, and Ser704) immediately postexercise (IPEX) and/or 3 h postexercise (3hPEX). Epitrochlearis muscles isolated from sedentary and exercised (2-h swim exercise; studied IPEX and 3hPEX) rats were incubated with 2-deoxyglucose to determine GU (without insulin at IPEX; without or with insulin at 3hPEX). Muscles were also assessed for γ1-AMPK activity, γ3-AMPK activity, phosphorylated AMPK (pAMPK), and phosphorylated AS160 (pAS160). IPEX versus sedentary had greater γ3-AMPK activity, pAS160 (Ser588, Thr642, Ser704), and GU with unaltered γ1-AMPK activity. 3hPEX versus sedentary had greater γ3-AMPK activity, pAS160 Ser704, and GU with or without insulin; greater pAS160 Thr642only with insulin; and unaltered γ1-AMPK activity. These results using an in vivo exercise protocol that increased insulin-stimulated GU in rat skeletal muscle are consistent with the hypothesis that in vivo exercise-induced enhancement of γ3-AMPK activation and AS160 Ser704IPEX and 3hPEX are important for greater pAS160 Thr642and enhanced insulin-stimulated GU by skeletal muscle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|