Cloning and functional characterization of the bovine endothelin-converting enzyme-1a promoter
| Autor: | Hans-Dieter Orzechowski, Julia Lemmer, Heiko Funke-Kaiser, Claus-Michael Richter, Martin Paul, Steffen Theis |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Gene isoform
Untranslated region Transcriptional Activation Molecular Sequence Data Biophysics Biology Endothelin-Converting Enzymes Transfection Biochemistry Start codon Structural Biology Consensus Sequence Genetics Animals Aspartic Acid Endopeptidases Cloning Molecular Luciferases Promoter Regions Genetic Transcription factor Cells Cultured Reporter gene Messenger RNA Base Sequence Reverse Transcriptase Polymerase Chain Reaction Metalloendopeptidases Promoter Nuclease protection assay Molecular biology Cattle Endothelium Vascular |
| Zdroj: | Biochimica et biophysica acta. 1446(3) |
| ISSN: | 0006-3002 |
| Popis: | Endothelin-converting enzyme-1 (ECE-1) mRNA is expressed in three isoforms, termed a, b, and c, originating from alternative promoters. In cultured bovine aortic endothelial cells, we detected mRNA isoform expression of ECE-1a and ECE-1b/c, respectively. Investigating transcriptional mechanisms of bovine endothelial ECE-1a expression in more detail, we identified multiple transcription start sites localized 120–415 nucleotides upstream from the presumptive translation start codon by RNase protection assay and 5′ RACE. Using luciferase reporter gene assays we found that 1.4 kb of the 5′ untranslated region showed strong promoter activity in endothelial cells. Sequence analysis revealed 71% overall homology of the bovine ECE-1a promoter with its human homologue. The proximal 680 base pair promoter region was shown to contain cis elements that are sufficient for basal and serum-induced transcriptional activation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|