Allergen-Specific MHC Class II Tetramer+ Cells Are Detectable in Allergic, but Not in Nonallergic, Individuals
| Autor: | Grete Sønderstrup, William W. Kwok, Jan Wahlstrom, Ana Paula Galvão da Silva, Dale T. Umetsu, Claudia Macaubas, Thomas G. Forsthuber, Rosemarie H. DeKruyff |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
CD4-Positive T-Lymphocytes
Genetically modified mouse Genes MHC Class II Molecular Sequence Data Immunology Mice Transgenic In Vitro Techniques Lymphocyte Activation Major histocompatibility complex medicine.disease_cause Epitope Mice Allergen Tetramer Antigen Hypersensitivity medicine Animals Humans Immunology and Allergy Amino Acid Sequence Protein Structure Quaternary Plant Proteins Mice Inbred BALB C MHC class II biology Immunodominant Epitopes Histocompatibility Antigens Class II food and beverages HLA-DR Antigens Allergens Antigens Plant Solubility biology.protein Cytokines Function (biology) HLA-DRB1 Chains |
| Zdroj: | ResearcherID |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.176.8.5069 |
| Popis: | Allergen-specific cells are present in very low frequency in peripheral blood of humans, and differ in function in allergic and nonallergic individuals. We report in this study that soluble class II MHC tetramers can be used to directly identify and study such allergen epitope-specific CD4+ T cells in humans. We identified the major antigenic epitope of rye grass allergen Lol p 1 in HLA-DRB1*0401 individuals using HLA-DR*0401 transgenic mice and peripheral blood cells from HLA-DR*0401 individuals. Using DRB1*0401 tetramers loaded with this major epitope of Lol p 1, we detected allergen-specific CD4+ T cells in the peripheral blood of DRB1*0401 rye grass allergic individuals after ex vivo expansion with allergen. These tetramer-positive cells produced IL-4, but little IFN-γ. In contrast, we were unable to detect rye grass tetramer-positive cells in cultures from HLA-DR*0401 nonallergic individuals, even after expansion with IL-2. Thus, our results suggest that rye grass allergen-specific T cells in DR*0401 nonallergic subjects are present at very low levels (e.g., because of deletion or suppression), differ in a fundamental way in their requirement for ex vivo expansion (e.g., they may be anergic), or use TCRs distinct from those of allergic individuals. Thus, analysis using DRB1*0401 tetramers loaded with a major epitope of Lol p 1 indicates that allergen-specific CD4+ T cells in nonallergic individuals are distinct from those in allergic subjects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|