Scavenging free radicals and soaring osteoinduction by extra cellular matrix protein-based nanocomposites on degenerative bone treatments
| Autor: | Vinoth Amirthalingam, Kavitha Kandiah, Balagurunathan Ramasamy, Navaneethan Duraisamy |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Antioxidant Free Radicals Thiobarbituric acid medicine.medical_treatment Bioengineering Nanotechnology 02 engineering and technology Bone and Bones Nitric oxide Nanocomposites Biomaterials Chitosan 03 medical and health sciences chemistry.chemical_compound medicine Chondroitin Bone regeneration Titanium Nanocomposite technology industry and agriculture Biomaterial 021001 nanoscience & nanotechnology Extracellular Matrix 030104 developmental biology chemistry Biochemistry Mechanics of Materials 0210 nano-technology |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 77 |
| ISSN: | 1873-0191 |
| Popis: | A number of materials are now available to alleviate the ever-growing bone disruption. However, these are inadequate and inappropriate for addressing issues associated natural process of aging and degeneration of bone due to diseases. This study advances the existing material and offers more privileged and synergistically active remedy for these conditions. Here, they are three different nano-composites prepared such as nano-TiO2 with chitosan (TC), nano-TiO2 with chondroitin 4-sulfate (TG), and nano-TiO2 with chitosan and chondroitin 4-sulfate (TCG), whereas nano-TiO2 act as a control. The prepared nanocomposite was studied for determining its bactericidal and fungicidal activity by using disk diffusion method. In addition, the osteoinductive, free radical forming, and scavenging abilities of the nanocomposite treated MG-63 cell lines were analyzed using gene expression and biochemical analysis respectively. The augmented fungicidal (~16mm) activities of TCG against bone-infecting pathogens can be effectively used in bone transplantation application. The expression of osteoblast-inducing genes in MG-63 cell line and their up-regulation in nanocomposite treatment, especially in TCG, made this material more desirable. The formation of free radicals such as thiobarbituric acid reactive substance and nitric oxide gradually reduced with the treatment of nanocomposites than control and nano-TiO2. Contrarily, it was found that MG-63 along with nanocomposites statistically increases the production of ALP, antioxidant enzymes (super oxide mutase) and total antioxidant activity (ferric reducing antioxidant power) in several folds compare with the control and nano-TiO2. All the results with statistical scale suggest TCG as an effectual and affordable biomaterial in bone regeneration therapy among the prepared samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect