Nitroreduction of 2,4-dinitrotoluene in vitro by cytochrome P-450 induced H4IIE cells
| Autor: | Wayne R. Mitchell, Elizabeth P. Burrows |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Carcinoma
Hepatocellular Environmental Engineering Cytochrome Cell Survival Health Toxicology and Mutagenesis Cytochrome P-450 Enzyme System Tumor Cells Cultured Animals Environmental Chemistry Inducer Enzyme inducer Benzofurans chemistry.chemical_classification Unspecific monooxygenase biology Mutagenicity Tests Chemistry Liver Neoplasms Public Health Environmental and Occupational Health Cytochrome P450 General Medicine General Chemistry Metabolism Pollution Rats Dinitrobenzenes Enzyme Biochemistry Cell culture Enzyme Induction Luminescent Measurements biology.protein Spectrophotometry Ultraviolet Aryl Hydrocarbon Hydroxylases Oxidation-Reduction |
| Zdroj: | Chemosphere. 31:2767-2777 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/0045-6535(95)00141-t |
| Popis: | Conditions have been established for H4IIE rat hepatoma cell cultures in which effects of cytochrome P-450 induction on the metabolism of a munitions wastestream pollutant can be studied. Under these conditions, the polychlorinated hydrocarbon 2,3,4,7,8-pentachlorodibenzfuran (PCDBF) induced cytochrome P-450 (1A1) aryl hydrocarbon hydroxylase (AHH) activity over a wide range of concentrations without significant cytotoxic effects. The munition pollutant 2,4-dinitrotoluene (2,4-DNT) did not induce AHH activity itself, but its metabolism was considerably altered when applied to PCDBF induced cultures. Production of amino nitrotoluene isomers was greatly enhanced in induced cultures as compared to uninduced controls, as was the conversion of radiolabeled 2,4-DNT to relatively more polar metabolites. To some extent, the results with H4IIE cells parallel those reported for animals exposed to 2,4-DNT after induction of cytochrome P-450 AHH activity. The preliminary findings suggest that with further development and validation, H4IIE cultures could be of use in characterizing metabolites that result from exposure to chemical mixtures involving a P-450 (1A1) inducer. |
| Databáze: | OpenAIRE |
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