Quantification of Nanoparticles in Dispersions Using Transmission Electron Microscopy
| Autor: | Ralf Kaegi, Bodo Hattendorf, Martin Fierz |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Particle number 010401 analytical chemistry technology industry and agriculture Electrostatic precipitator Nanoparticle 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Nanomaterials Deposition (aerosol physics) Chemical engineering Transmission electron microscopy Sample preparation Particle size 0210 nano-technology Instrumentation |
| Zdroj: | Microscopy and Microanalysis. 27:557-565 |
| ISSN: | 1435-8115 1431-9276 |
| Popis: | The quantification of the particle size and the number concentration (PNC) of nanoparticles (NPs) is key for the characterization of nanomaterials. Transmission electron microscopy (TEM) is often considered as the gold standard for assessing the size of NPs; however, the TEM sample preparation suitable for estimating the PNC based on deposited NPs is challenging. Here, we use an ultrasonic nebulizer (USN) to transfer NPs from aqueous suspensions into dried aerosols which are deposited on TEM grids in an electrostatic precipitator of an aerosol monitor. The deposition efficiency of the electrostatic precipitator was ≈2%, and the transport efficiency of the USN was ≈7%. Experiments using SiO2 NPs (50–200 nm) confirmed an even deposition of the nebulized particles in the center of the TEM grids. PNCs of the SiO2 NPs derived from TEM images underestimated the expected PNCs of the suspensions by a factor of up to three, most likely resulting from droplet coagulation and NP aggregation in the USN. Nevertheless, single particles still dominated the PNC. Our approach results in reproducible and even deposition of particles on TEM grids suitable for morphological analysis and allows an estimation of the PNC in the suspensions based on the number of particles detected by TEM. |
| Databáze: | OpenAIRE |
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