Nipah virus induces two inclusion body populations: Identification of novel inclusions at the plasma membrane
Autor: | Lucie Sauerhering, Bevan Sawatsky, Sandro Halwe, Laura Behner, Marc Ringel, Nico Becker, Andrea Maisner, Erik Dietzel, Anja Heiner, Larissa Kolesnikova |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Paramyxoviridae
QH301-705.5 Immunology Population Biology Microbiology Virus Inclusion bodies Inclusion Bodies Viral Viral Matrix Proteins 03 medical and health sciences Virology Chlorocebus aethiops Genetics Animals Humans Biology (General) education Mononegavirales Molecular Biology Vero Cells 030304 developmental biology Glycoproteins Henipavirus Infections 0303 health sciences education.field_of_study Virus Assembly 030302 biochemistry & molecular biology Cell Membrane Nipah Virus RC581-607 Virus Internalization biology.organism_classification Pneumoviridae Aggresome Membrane protein Parasitology Immunologic diseases. Allergy Research Article |
Zdroj: | PLoS Pathogens PLoS Pathogens, Vol 15, Iss 4, p e1007733 (2019) |
ISSN: | 1553-7374 1553-7366 |
Popis: | Formation of cytoplasmic inclusion bodies (IBs) is a hallmark of infections with non-segmented negative-strand RNA viruses (order Mononegavirales). We show here that Nipah virus (NiV), a bat-derived highly pathogenic member of the Paramyxoviridae family, differs from mononegaviruses of the Rhabdo-, Filo- and Pneumoviridae families by forming two types of IBs with distinct localizations, formation kinetics, and protein compositions. IBs in the perinuclear region form rapidly upon expression of the nucleocapsid proteins. These IBperi are highly mobile and associate with the aggresome marker y-tubulin. IBperi can recruit unrelated overexpressed cytosolic proteins but do not contain the viral matrix (M) protein. Additionally, NiV forms an as yet undescribed IB population at the plasma membrane (IBPM) that is y-tubulin-negative but contains the M protein. Infection studies with recombinant NiV revealed that IBPM require the M protein for their formation, and most likely represent sites of NiV assembly and budding. The identification of this novel type of plasma membrane-associated IBs not only provides new insights into NiV biology and may open new avenues to develop novel antiviral approaches to treat these highly pathogenic viruses, it also provides a basis for a more detailed characterization of IBs and their role in virus assembly and replication in infections with other Mononegavirales. Author summary Inclusion bodies (IBs) induced by non-segmented negative-strand RNA viruses (Mononegavirales) are described as mobile cytosolic compartments that concentrate viral proteins and represent the main viral replication sites in infected cells. This general concept is mainly based on studies with mononegaviruses from the Rhabdo-, Filo- and Pneumoviridae families. IBs induced by members of the Paramyxoviridae family are much less well characterized, and this study provides evidence that paramyxoviral IBs may have different compositions and functions. The main finding of this study is that Nipah virus (NiV), a highly pathogenic member of the genus Henipavirus in the family Paramyxoviridae, forms a novel type of IB whose formation at plasma membrane assembly sites depends on the viral matrix protein, and suggests a role for IBs not yet described for other Mononegavirales. This discovery clearly extents the current concept of IB functions and illustrates the need to further investigate IBs formed by other paramyxoviruses. |
Databáze: | OpenAIRE |
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