The evolutionary history of the HUP domain
| Autor: | Jagoda Jabłońska, Liam M. Longo, Ita Gruić-Sovulj, Dan S. Tawfik |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Nucleotides Aminoacyl tRNA synthetase Ribose Last universal ancestor Computational biology Biochemistry HIGH motif PP-ATPase aminoacyl-tRNA synthetases Rossmannoid nucleotide binding domain protein evolution last universal common ancestor Amino Acyl-tRNA Synthetases Evolution Molecular chemistry.chemical_compound Enzyme chemistry Cyclic nucleotide-binding domain Nucleotide Amino Acid Sequence NAD+ kinase Sequence Alignment Molecular Biology Adenylylation |
| Zdroj: | Critical Reviews in Biochemistry and Molecular Biology. 57:1-15 |
| ISSN: | 1549-7798 1040-9238 |
| DOI: | 10.1080/10409238.2021.1957764 |
| Popis: | Among the enzyme lineages that undoubtedly emerged prior to the last universal common ancestor is the so-called HUP, which includes Class I aminoacyl tRNA synthetases (AARSs) as well as enzymes mediating NAD, FAD, and CoA biosynthesis. Here, we provide a detailed analysis of HUP evolution, from emergence to structural and functional diversification. The HUP is a nucleotide binding domain that uniquely catalyzes adenylation via the release of pyrophosphate. In contrast to other ancient nucleotide binding domains with the αβα sandwich architecture, such as P-loop NTPases, the HUP’s most conserved feature is not phosphate binding, but rather ribose binding by backbone interactions to the tips of β1 and/or β4. Indeed, the HUP exhibits unusual evolutionary plasticity and, while ribose binding is conserved, the location and mode of binding to the base and phosphate moieties of the nucleotide, and to the substrate(s) reacting with it, have diverged with time, foremost along the emergence of the AARSs. The HUP also beautifully demonstrates how a well- packed scaffold combined with evolvable surface elements promotes evolutionary innovation. Finally, we offer a scenario for the emergence of the HUP from a seed βαβ fragment, and suggest that despite an identical architecture, the HUP and the Rossmann represent independent emergences. |
| Databáze: | OpenAIRE |
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