Elevated IGFBP3 Levels in Diabetic Tears: A Negative Regulator of IGF-1 Signaling in the Corneal Epithelium
| Autor: | Danielle M. Robertson, Benjamin R. Buckner, H. Dwight Cavanagh, Meifang Zhu, Yu-Chieh Wu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Blotting Western IGFBP3 Enzyme-Linked Immunosorbent Assay Biology Real-Time Polymerase Chain Reaction Article Corneal Diseases Receptor IGF Type 1 In vivo Internal medicine Diabetes mellitus Cornea medicine Humans Insulin-Like Growth Factor I Cells Cultured Corneal epithelium Epithelium Corneal Middle Aged medicine.disease Ophthalmology Diabetes Mellitus Type 1 Insulin-Like Growth Factor Binding Protein 3 medicine.anatomical_structure Endocrinology Real-time polymerase chain reaction Diabetes Mellitus Type 2 Tears Female Mannitol medicine.drug |
| Zdroj: | The Ocular Surface. 10:100-107 |
| ISSN: | 1542-0124 |
| DOI: | 10.1016/j.jtos.2012.01.004 |
| Popis: | To determine the ratio of IGFBP3:IGF-1 in normal and diabetic human tears, and in telomerase-immortalized human corneal epithelial cells (hTCEpi) cultured under elevated glucose conditions and to correlate these changes with total and phosphorylated levels of IGF-1R. Tear samples were collected noninvasively from diabetic subjects and non-diabetic controls; corneal sensitivity was assessed using a Cochet-Bonnet Aesthesiometer. Conditioned media were collected following culture of hTCEpi cells in normal (5 mM) and elevated (25 mM) glucose conditions; mannitol was used as an osmotic control. IGFBP3, IGF-1, and phosphorylated IGF-1R levels were assessed by ELISA. IGFBP3 and IGF-1R mRNA were assessed by real-time polymerase chain reaction (PCR). Total and phosphorylated IGF-1R expression in whole cell lysates was assessed by western blot. There was a 2.8-fold increase in IGFBP3 in diabetic tears compared to non-diabetic controls (P=0.006); IGF-1 levels were not significantly altered. No difference in corneal sensitivity was detected between groups. The concentration of IGFBP3 in tears was independent of IGF-1. Consistent with human tear measurements in vivo, IGFBP3 secretion was increased 2.2 fold (P |
| Databáze: | OpenAIRE |
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