Immunohistochemical Detection of Avian Pneumovirus in Formalin-Fixed Tissues
| Autor: | David A. Halvorson, Faris F. Jirjis, Kakambi V. Nagaraja, Daniel P. Shaw, Sally Noll |
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| Rok vydání: | 2001 |
| Předmět: |
Male
0301 basic medicine Turkeys Pathology medicine.medical_specialty Tissue Fixation 040301 veterinary sciences 030106 microbiology Viral antigen Biology 0403 veterinary science 03 medical and health sciences Antigen Formaldehyde Submucosa otorhinolaryngologic diseases medicine Animals Pneumovirus Infections Antigens Viral Poultry Diseases Nasal Turbinate Pneumovirus General Veterinary 04 agricultural and veterinary sciences Formalin fixed Immunohistochemistry Avian pneumovirus Nasal Mucosa Tissue sections medicine.anatomical_structure Female Rabbits |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 13:13-16 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/104063870101300103 |
| Popis: | An immunohistochemical staining technique (IHC) was developed to detect avian pneumovirus (APV) antigen in formalin-fixed, paraffin-embedded tissue sections using streptavidin-biotin immunoperoxidase staining. Samples of nasal turbinates and infraorbital sinuses were collected from 4-week-old poults experimentally inoculated with APV and from older turkeys infected during naturally occurring outbreaks of avian pneumovirus. Tissue was fixed in 10% buffered neutral formalin, embedded in paraffin, sectioned and stained. Inflammatory changes were observed microscopically in the mucosa and submucosa of the nasal turbinates and infraorbital sinuses of both experimentally inoculated poults and naturally infected birds. Viral antigen was detected by IHC in the ciliated epithelial cells of nasal turbinates and infraorbital sinuses. |
| Databáze: | OpenAIRE |
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