The distribution of conjunctival goblet cells in mice
| Autor: | Friedrich Paulsen, Jessica Feldt, Jessica Welss, Nichapa Punchago |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Conjunctiva Biology Lacrimal apparatus law.invention Mice 03 medical and health sciences chemistry.chemical_compound law medicine Microtome Animals Distribution (pharmacology) Paraformaldehyde Goblet cell Mucin Eyelids General Medicine respiratory system eye diseases Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure chemistry Dry Eye Syndromes Goblet Cells sense organs 030101 anatomy & morphology Eyelid Anatomy Developmental Biology |
| Zdroj: | Annals of Anatomy - Anatomischer Anzeiger. 234:151664 |
| ISSN: | 0940-9602 |
| Popis: | Purpose To evaluate the density and distribution of conjunctival goblet cells in mice without clinical evidence of ocular surface diseases. Methods Immediately after euthanasia of C57BL/6 wild-type mice, the eyes including eyelids were removed and fixed in paraformaldehyde. Entire eyeballs and eyelids were cut in series along the sagittal axis from nasal to temporal on a microtome and then stained with Periodic Acid-Schiff acid to visualize the goblet cells. At each section stained in this way, the conjunctival goblet cells of the entire upper and lower lid conjunctiva were counted by light microscopy. Additional (transmission electron microscopy) (TEM)-Analysis on ultrathin sections was performed to evaluate morphological differences. Results The total number of conjunctival goblet cells differs markedly between individual animals. Categorisation into upper eyelid (UL) and lower eyelid (LL) and into regions (nasal, middle, temporal) revealed a significant increase of goblet cells from nasal to temporal in the UL and a significant decrease in the LL. Conclusion The distribution of conjunctival goblet cells in mice differs considerably from humans and between individual animals. Therefore, precise selection of sampling and methods are needed to obtain comparable data. We recommend to use the middle region of the conjunctiva of UL/LL for goblet cell studies in mice. These findings are of particular interest for dry eye mouse models as well as pharmacological studies on mice with influence on their goblet cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect