Anti-Inflammatory Activity Is a Possible Mechanism by Which the Polyherbal Formulation Comprised ofNigella sativa(Seeds),Hemidesmus indicus(Root), andSmilax glabra(Rhizome) Mediates Its Antihepatocarcinogenic Effects

Autor: M. Ira Thabrew, G. A. K. Weerasinghe, M G Thammitiyagodage, Kamani H. Tennekoon, Sameera R. Samarakoon, W.D. Ratnasooriya, Prasanna B Galhena
Rok vydání: 2012
Předmět:
Zdroj: Evidence-based Complementary and Alternative Medicine : eCAM
Evidence-Based Complementary and Alternative Medicine, Vol 2012 (2012)
ISSN: 1741-4288
1741-427X
Popis: The present study investigated the anti-inflammatory effects of a polyherbal decoction comprised ofNigella sativa, Hemidesmus indicus, andSmilax glabrain order to justify its claimed antihepatocarcinogenic activity. Activation of hepatic nuclear factor-kappa B (NF-κB), IκB kinase (IKKα/β) proteins, and TNFαand IL-6 expression was investigated in diethylnitrosamine- (DEN-) induced C3H mice-bearing early hepatocarcinogenic changes. Acute phase inflammatory response was evaluated by carrageenan-induced rat paw edema formation. Anti-inflammatory mechanisms were also assessed by determining effect on (a) membrane stabilization, (b) nitric oxide (NO) inhibitory activity, and (c) inhibition of leukocyte migration. A significant inhibition of the paw edema formation was observed in healthy rats as well as in rats bearing early hepatocarcinogenic changes with oral administration of the decoction. As with the positive control, indomethacin (10 mg/kg b.w.) the inhibitory effect was pronounced at 3rd and 4th h after carrageenan injection. A notable IKKα/βmediated hepatic NF-κB inactivation was associated with a significant hepatic TNFαdownregulation among mice-bearing hepatocarcinogenic changes subjected to decoction treatment. Inhibition of NO production, leukocyte migration, and membrane stabilization are possible mechanisms by which anti-inflammatory effect is mediated by the decoction. Overall findings imply that anti-inflammatory activity could be one of the mechanisms by which the decoction mediates its antihepatocarcinogenic effects.
Databáze: OpenAIRE
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