Platelet-derived growth factor BB mediated regulation of 12-lipoxygenase in porcine aortic smooth muscle cells
| Autor: | Vaijayanthy Rangarajan, Wei Bai, Rama Natarajan, Jia-Li Gu, Linda Lanting, Noe Gonzales, Jerry L. Nadler |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Physiology Swine medicine.medical_treatment Clinical Biochemistry Blotting Western Arachidonate 12-Lipoxygenase Lipoxygenase Cell Movement Internal medicine medicine Animals Cyclooxygenase Inhibitors 12-Hydroxy-5 8 10 14-eicosatetraenoic Acid RNA Messenger Enzyme Inhibitors Aorta Cells Cultured Flavonoids Platelet-Derived Growth Factor biology Growth factor Chemotaxis Muscle Smooth Cell Biology Blotting Northern Angiotensin II Endocrinology Glucose Gene Expression Regulation Cell culture Flavanones biology.protein Cyclooxygenase Platelet-derived growth factor receptor Cell Division |
| Zdroj: | Journal of cellular physiology. 169(2) |
| ISSN: | 0021-9541 |
| Popis: | Platelet-derived growth factor BB (PDGF) is a potent mitogen and chemoattractant for vascular smooth muscle cells (VSMC). In the present study, we have examined the effects of PDGF on the 12-lipoxygenase (12-LO) pathway of arachidonate metabolism in porcine aortic VSMC (PVSMC). The rationale for this is previous studies showing that LO products have growth and chemotactic effects in VSMC and that another VSMC growth factor, angiotensin II, is a potent positive regulator of 12-LO activity and expression. We observed that PDGF causes a significant increase in the formation of the 12-LO product, 12-hydroxyeicosatetraenoic acid (12-HETE) in PVSMC. In addition, PDGF also markedly increased leukocyte-type 12-LO messenger RNA and protein expression. PDGF-induced PVSMC migration was inhibited significantly by two LO blockers but not by a cyclooxygenase blocker. Furthermore, although the proliferative effects of PDGF on PVSMC were not altered by cell culture under hyperglycemic conditions (25 mM glucose, HG), the chemotactic effects of PDGF as well as those of 10% fetal calf serum were significantly greater in cells cultured in HG as compared to normal glucose conditions (5.5 mM), thus indicating a potential new mechanism for the accelerated cardiovascular disease usually observed in diabetes. These results indicate a novel mechanism for the biological effects of PDGF in leading to cardiovascular disease. © 1996 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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