The Development of a Loop-Mediated Isothermal Amplification Method (LAMP) for Echinococcus granulosis Coprodetection
| Autor: | Harold Salant, Joseph Hamburger, Ibrahim Abbasi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Loop-mediated isothermal amplification
Biology Echinococcus multilocularis Sensitivity and Specificity law.invention Feces chemistry.chemical_compound law Virology parasitic diseases Animals Humans Echinococcus granulosus Parasite Egg Count Polymerase chain reaction DNA Primers Base Sequence Articles Nucleic acid amplification technique DNA Protozoan Amplicon biology.organism_classification Molecular biology Infectious Diseases Echinococcus chemistry Parasitology Nucleic Acid Amplification Techniques DNA |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 87:883-887 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.2012.12-0184 |
| Popis: | We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare. |
| Databáze: | OpenAIRE |
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