Leptospira : Molecular Detection of Pathogenic Species in Natural Sources
| Autor: | Brittney Beigel, Ashutosh Verma |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Lipoproteins 030106 microbiology Biology Real-Time Polymerase Chain Reaction Microbiology Rapid detection 03 medical and health sciences chemistry.chemical_compound Environmental water Leptospira Virology medicine TaqMan Gene Bacteriological Techniques General Medicine medicine.disease biology.organism_classification Leptospirosis Real-time polymerase chain reaction chemistry Parasitology Water Microbiology DNA Bacterial Outer Membrane Proteins |
| Zdroj: | Current Protocols in Microbiology |
| ISSN: | 1934-8533 1934-8525 |
| DOI: | 10.1002/cpmc.43 |
| Popis: | This protocol describes a method for the rapid detection of leptospiral DNA in environmental water. In summary, the DNA is extracted from water samples and tested in a TaqMan-based real-time quantitative polymerase chain reaction (qPCR) for the presence of lipl32, a gene that is present only in pathogenic Leptospira spp. The gene target used in this assay is important in that it only detects pathogenic leptospires and not the saprophytic leptospires that may be present in environmental samples. © 2017 by John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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