d -Glucose-Recognition and Phlorizin-Binding Sites in Human Sodium/d -Glucose Cotransporter 1 (hSGLT1): A Tryptophan Scanning Study
| Autor: | Dharmendra Pandey, Pankaj Goyal, Navneet K. Tyagi, Wolfgang Siess, Rolf K. H. Kinne, Azad Kumar |
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| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Phlorizin Phloretin Proteolipids Mutant Biochemistry Pichia Structure-Activity Relationship chemistry.chemical_compound Sodium-Glucose Transporter 1 D-Glucose Humans Binding site Liposome Binding Sites Ion Transport Tryptophan Kinetics Glucose Phlorhizin Spectrometry Fluorescence chemistry Liposomes Mutation Mutagenesis Site-Directed Cotransporter |
| Zdroj: | Biochemistry. 46:13616-13628 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi701193x |
| Popis: | In order to gain a better understanding of the structure-function relation in hSGLT1, single Trp residues were introduced into a functional hSGLT1 mutant devoid of Trps at positions that previously had been postulated to be involved in sugar recognition/translocation and/or phlorizin binding. The mutant proteins were expressed in Pichia pastoris, purified, and reconstituted into liposomes. In transport experiments the putative sugar binding site mutants W457hSGLT1 and W460hSGLT1 showed a drastic decrease in affinity toward alpha-methyl-d-glucopyranoside with Km values of 13.3 and 5.26 mM compared to 0.4 mM of the Trp-less hSGLT1. In addition, a strong decrease in the inhibitory effect of phlorizin was observed. In Trp fluorescence studies the position of the emission maxima of the mutants, their sensitivity to N-bromosuccinimide oxidation, and their interaction with water soluble quenchers demonstrate that Trp457 and Trp460 are in contact with the hydrophilic extravesicular environment. In both mutants Trp fluorescence was quenched significantly, but differently, by various glucose analogues. They also show significant protection by d-glucose and phlorizin against acrylamide, KI, or TCE quenching. W602hSGLT1 and W609hSGLT1, the putative aglucone binding site mutants, exhibit normal sugar and phlorizin affinity, and show fluorescence properties which indicate that these residues are located in a very hydrophilic environment. Phlorizin and phloretin, but not d-glucose, protect both mutants against collisional quenchers. Depth-calculations using the parallax method suggest a location of Trp457 and Trp460 at an average distance of 10.8 A and 7.4 A from the center of the bilayer, while Trp602 and Trp609 are located outside the membrane. These results suggest that in the native carrier residues Gln at position 457 and Thr at position 460 reside in a hydrophilic access pathway extending 5-7 A into the membrane to which sugars as well as the sugar moiety of inhibitory glucosides bind. Residues Phe602 and Phe609 contribute by their hydrophobic aromatic residues toward binding of the aglucone part of phlorizin. Thereby in the phlorizin-carrier complex a close vicinity between these two subdomains of the transporter is established creating a phlorizin binding pocket with the previously estimated dimensions of 10 x 17 x 7 A. |
| Databáze: | OpenAIRE |
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