Antimicrobial Susceptibility Test with Plasmonic Imaging and Tracking of Single Bacterial Motions on Nanometer Scale
| Autor: | Rafael Iriya, Shelley E. Haydel, Shaopeng Wang, Karan Syal, Hui Yu, Yunze Yang, Nongjian Tao, Hong-Yuan Chen |
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| Rok vydání: | 2015 |
| Předmět: |
ANTIMICROBIAL SUSCEPTIBILITY TESTS
medicine.drug_class Surface Properties Antibiotics General Physics and Astronomy Antimicrobial susceptibility 02 engineering and technology Microbial Sensitivity Tests Biology digestive system 01 natural sciences Narrow spectrum Microbiology Motion medicine Escherichia coli General Materials Science 010401 analytical chemistry Optical Imaging General Engineering Cells Immobilized Surface Plasmon Resonance 021001 nanoscience & nanotechnology digestive system diseases 0104 chemical sciences 3. Good health Anti-Bacterial Agents Clinical microbiology 0210 nano-technology Healthcare providers |
| Zdroj: | ACS nano. 10(1) |
| ISSN: | 1936-086X |
| Popis: | Antimicrobial susceptibility tests (ASTs) are important for confirming susceptibility to empirical antibiotics and detecting resistance in bacterial isolates. Currently, most ASTs performed in clinical microbiology laboratories are based on bacterial culturing, which take days to complete for slowly growing microorganisms. A faster AST will reduce morbidity and mortality rates and help healthcare providers administer narrow spectrum antibiotics at the earliest possible treatment stage. We report the development of a nonculture-based AST using a plasmonic imaging and tracking (PIT) technology. We track the motion of individual bacterial cells tethered to a surface with nanometer (nm) precision and correlate the phenotypic motion with bacterial metabolism and antibiotic action. We show that antibiotic action significantly slows down bacterial motion, which can be quantified for development of a rapid phenotypic-based AST. |
| Databáze: | OpenAIRE |
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