How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid:ribonucleic acid duplex

Autor:	Margriet Ovaere, Piet Herdewijn, Judit E. Šponer, Luc Van Meervelt, Jiri Sponer
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	Models Molecular Messenger RNA Small interfering RNA Stereochemistry Base pair Hydrogen bond RNA Water Hydrogen Bonding Biology Sugar Alcohols Biochemistry RNA interference Duplex (building) Structural Biology Genetics Nucleic acid Nucleic Acid Conformation RNA Small Interfering RNA Double-Stranded
Zdroj:	Nucleic Acids Research
ISSN:	1362-4962 0305-1048
Popis:	Altritol nucleic acids (ANAs) are a promising new tool in the development of artificial small interfering ribonucleic acids (siRNAs) for therapeutical applications. To mimic the siRNA:messenger RNA (mRNA) interactions, the crystal structure of the ANA:RNA construct a(CCGUAAUGCC-P):r(GGCAUUACGG) was determined to 1.96 Å resolution which revealed the hybrid to form an A-type helix. As this A-form is a major requirement in the RNAi process, this crystal structure confirms the potential of altritol-modified siRNAs. Moreover, in the ANA strands, a new type of intrastrand interactions was found between the O2' hydroxyl group of one residue and the sugar ring O4' atom of the next residue. These interactions were further investigated by quantum chemical methods. Besides hydration effects, these intrastrand hydrogen bonds may also contribute to the stability of ANA:RNA duplexes. ispartof: Nucleic Acids Research vol:40 issue:15 pages:7573-7583 ispartof: location:England status: published
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3be8b4eb53f389f6c29547cc8ad5fa5a http://europepmc.org/articles/PMC3424580 Zobrazit plný text záznamu