Insight into the drug resistance whole genome of Mycobacterium tuberculosis isolates from Khyber Pakhtunkhwa, Pakistan
| Autor: | Aman Chandra Kaushik, Shulin Zhang, Arif Ali, Heng Wang, Abbas Khan, Amie Jinghua Wei, Muhammad Irfan, Zhilei Cui, Sathishkumar Chinnasamy, Sajid Ali, Yu-Juan Zhang, Ming-Zhu Zhao, Anwar Khan, Dong-Qing Wei, Muhammad Tahir Khan, Kejia Liu, Yanjie Wang, Muhammad Tariq Zeb |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Drug Tuberculosis media_common.quotation_subject 030106 microbiology Virulence Drug resistance Microbial Sensitivity Tests Microbiology Genome Mycobacterium tuberculosis 03 medical and health sciences Drug Resistance Multiple Bacterial Tuberculosis Multidrug-Resistant Genetics medicine Humans Pakistan Molecular Biology Ecology Evolution Behavior and Systematics media_common Whole genome sequencing biology Whole Genome Sequencing biology.organism_classification medicine.disease 030104 developmental biology Infectious Diseases Mycobacterium tuberculosis complex Mutation Genome Bacterial |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 92 |
| ISSN: | 1567-7257 |
| Popis: | Whole genome sequencing (WGS) is one of the most reliable methods for detection of drug resistance, genetic diversity in other virulence factor and also evolutionary dynamics of Mycobacterium tuberculosis complex (MTBC). First-line anti-tuberculosis drugs are the major weapons against Mycobacterium tuberculosis (MTB). However, the emergence of drug resistance remained a major obstacle towards global tuberculosis (TB) control program 2030, especially in high burden countries including Pakistan. To overcome the resistance and design potent drugs, genomic variations in drugs targets as well as in the virulence and evolutionary factors might be useful for better understanding and designing potential inhibitors. Here we aimed to find genomic variations in the first-line drugs targets, along with other virulence and evolutionary factors among the circulating isolates in Khyber Pakhtunkhwa, Pakistan. Samples were collected and drug susceptibility testing (DST) was performed as per WHO standard. The resistance samples were subjected to WGS. Among the five whole genome sequences, three samples (NCBI BioProject Accession: PRJNA629298, PRJNA629388) harbored 1997, 1162, and 2053 mutations. Some novel mutations have been detected in drugs targets. Similarly, numerous novel variants have also been detected in virulency and evolutionary factors, PE, PPE, and secretory system of MTB isolates. Exploring the genomic variations among the circulating isolates in geographical specific locations might be useful for future drug designing. To the best of our knowledge, this is the first study that provides useful data regarding the insight genomic variations in virulency, evolutionary factors including ESX and PE/PPE as well as drug targets, for better understanding and management of TB in a WHO declared high burden country. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect