Ovulatory and endocrine responses after active immunization of gilts against a synthetic fragment of bovine inhibin
| Autor: | James J. Ireland, W. L. Flowers, L. A. C. Sesti, B. F. King, T. L. Martin, K.L. Esbenshade, Jack H. Britt |
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| Rok vydání: | 1993 |
| Předmět: |
Ovulation
endocrine system medicine.medical_specialty Swine media_common.quotation_subject Immunization Secondary Endogeny Active immunization Gonadotropin-Releasing Hormone Estrus Internal medicine Genetics medicine Animals Endocrine system Inhibins Alpha globulin Progesterone media_common Estrous cycle Estradiol Chemistry Antibody titer General Medicine Luteinizing Hormone Endocrinology Pituitary Gland Antibody Formation Female Immunization Animal Science and Zoology Follicle Stimulating Hormone hormones hormone substitutes and hormone antagonists Food Science Hormone |
| Zdroj: | Journal of Animal Science. 71:975-982 |
| ISSN: | 1525-3163 0021-8812 |
| Popis: | The objective of this study was to determine whether neutralizing endogenous inhibin would affect ovulation rate and serum concentrations of FSH, LH, estradio1-17@, and progesterone in gilts. At wk 0, during their second postpuberal estrous cycle, gilts (195 C 2.4 d of age) were given a primary immunization against the 1-26 gly-tyr NH-terminal amino acid sequence of bovine inhibin-a conjugated to human alpha globulin (INH; n = 10) or against human alpha globulin alone (control; n = 10). The primary immunization mixed with Freund's complete adjuvant contained .915 mg of the inhibin peptide. Booster immunizations in Freund's incomplete adju- vant contained .3 and .183 mg of the inhibin peptide and were given at wk 8 and 12, respectively. Free, unconjugated inhibin was given to INH gilts at 16 wk. Blood samples for determination of hormones were collected every 4 h beginning on d 15 of the first estrous cycle beyond wk 16 (first cycle) and continu- ing until d 5 of the second estrous cycle following wk 16 (second cycle). Ovulation rate was estimated by laparoscopy during the second cycle. Antibody titers were estimated by determining the percentage of (1251)-INH bound by serum diluted 1:4,000. The antibody titers were 17 k 2, 22 C 3, and 9 k 1% at wk 9, 17, and 23 for INH gilts, respectively, and 0% at all times for control gilts. Duration of three consecutive estrous cycles terminating with the first experimental cycle did not differ between treatments (INH, 20.7 k .3 vs control, 20.4 k .3 d). Immunization against INH resulted in a 39% increase in ovulation rate (17.8 L- .9 vs 12.8 _+ .7; P < .Ol). There was no difference between treatment groups for serum profiles of LH, estradiol- 170, or progesterone, but there was a time x treatment interaction for concentrations of FSH. The FSH was higher in INH gilts than in control gilts before the ovulatory surge of LH and lower for INH gilts than for control gilts after the surge. In summary, gilts immunized against a synthetic peptide of bovine inhibin-cy had higher ovulation rates than controls and displayed a time x treatment interaction in the pattern of secretion of FSH, but not LH, estradiol-& or progesterone. |
| Databáze: | OpenAIRE |
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