Controlled encapsidation of gold nanoparticles by a viral protein shell
| Autor: | Veronica R. Basnayake, Richard H. Guenther, Stefan Franzen, Steven A. Lommel, Lina Loo |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Viral protein
viruses Metal Nanoparticles Nanotechnology medicine.disease_cause Biochemistry Catalysis Virus Colloid and Surface Chemistry Microscopy Electron Transmission Tombusviridae medicine biology Chemistry Oligonucleotide RNA General Chemistry biology.organism_classification Dianthovirus Cell biology Capsid DNA Viral Nucleic acid RNA Viral Capsid Proteins Gold |
| Zdroj: | Journal of the American Chemical Society. 128(14) |
| ISSN: | 0002-7863 |
| Popis: | Icosahedral virus capsids demonstrate a high degree of selectivity in packaging cognate nucleic acid components during assembly. This packaging specificity, when integrated as part of a nanotechnological protocol, has the potential to encapsidate a wide array of foreign materials for delivery of therapeutics or biosensors into target cells. Red clover necrotic mosaic virus (RCNMV) exclusively packages two genomic ssRNAs initiated by a specific protein:RNA interaction between the RCNMV coat protein (CP) and the viral RNA origin of assembly (OAS) element. In the present work, an oligonucleotide mimic of the RCNMV OAS sequences is attached to Au nanoparticles as a recognition signal to initiate the virion-like assembly by RCNMV CP. Covalent linkage of the OAS to Au functions as a trigger for specific encapsidation and demonstrates that foreign cargo can be packaged into RCNMV virions. |
| Databáze: | OpenAIRE |
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