Physical structure and absorption properties of tailor-made porous starch granules produced by selected amylolytic enzymes

Autor: Byung-Hoo Lee, Sang-Ho Yoo, Yi-seul Jung
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Confocal Microscopy
Starch
lcsh:Medicine
beta-Amylase
02 engineering and technology
Biochemistry
Starches
Reaction rate
chemistry.chemical_compound
Bacillus licheniformis
Amylase
lcsh:Science
Microscopy
Microscopy
Confocal
Multidisciplinary
Calorimetry
Differential Scanning
biology
Organic Compounds
Hydrolysis
Granule (cell biology)
Temperature
Chemical Reactions
Light Microscopy
04 agricultural and veterinary sciences
Plants
021001 nanoscience & nanotechnology
Lipids
040401 food science
Enzymes
Chemistry
Experimental Organism Systems
Physical Sciences
Aspergillus niger
0210 nano-technology
Alpha-amylase
Porosity
Research Article
Cognitive Neuroscience
Carbohydrates
Calorimetry
Research and Analysis Methods
Zea mays
Model Organisms
0404 agricultural biotechnology
Plant and Algal Models
Reaction Time
Grasses
Organic Chemistry
lcsh:R
Chemical Compounds
Organisms
Water
Biology and Life Sciences
Proteins
Hordeum
biology.organism_classification
Soybean Oil
Maize
Absorption
Physicochemical
chemistry
Enzyme Structure
Biocatalysis
Microscopy
Electron
Scanning
Enzymology
biology.protein
Cognitive Science
lcsh:Q
Glucan 1
4-alpha-Glucosidase
alpha-Amylases
Oils
Confocal Laser Microscopy
Neuroscience
Nuclear chemistry
Zdroj: PLoS ONE, Vol 12, Iss 7, p e0181372 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: Porous starch granules (PSGs) with various pores and cavity sizes were prepared by amylolysis enzymes. The greatest hydrolysis rate on corn starch granule was observed with α-amylase, followed by gluco- and β-amylases. Temperature increase enhanced glucoamylase reaction rate more drastically than other enzyme treatments. Final hydrolysis level with glucoamylase reached to 66.9%, close to 67.5% of α-amylolysis. The α-amylase-treated PSGs displayed the greatest pore size and ratio of cavity-to-granule diameters. Gelatinization onset temperatures of PSGs increased to 72.1 (α-), 68.7 (β-), and 68.1°C (gluco-amylolysis) after 8 h; enthalpy changes of β- and gluco-amylase-treated PSGs increased to 13.4, and 13.1 J/g but α-amylase-treated one showed slightly reduced value of 8.5 J/g. Water holding capacities of PSGs were 209.7 (α-), 94.6 (β-), and 133.8% (gluco-amylolysis), and the untreated control had 89.1%; oil holding capacities of them showed 304.5, 182.7, and 211.5%, respectively, while the untreated control had 161.8%. Thus, enzyme types and their reaction conditions can be applied to generate desirable cavity and pore sizes in starch granules. This biocatalytic approach could contribute to develop tailor-made PSGs with distinct internal structure for specific uses in wide range of food, pharmaceutical and other industrial applications.
Databáze: OpenAIRE
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