The expression of cockroach insulin-like peptides is differentially regulated by physiological conditions and affected by compensatory regulation
| Autor: | Marc Castells, Iamil Ferrer, José L. Maestro, Júlia Castro-Arnau, Ainoa Marín |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), European Commission, Generalitat de Catalunya |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nutritional signaling Physiology medicine.medical_treatment Fat Body Vitellogenin 01 natural sciences 03 medical and health sciences Gene expression medicine Animals Amino Acid Sequence Gene knockdown biology Insulin Ovary Vitellogenesis Brain Blattellidae Receptor Insulin Cell biology Juvenile Hormones 010602 entomology Insulin receptor Blattella germanica 030104 developmental biology Gene Expression Regulation Starvation Insect Science Juvenile hormone biology.protein Insect Proteins Female Insect Vitellogenins Insulin-like peptide Relaxin/insulin-like family peptide receptor 2 |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0022-1910 |
| DOI: | 10.1016/j.jinsphys.2019.02.010 |
| Popis: | In insects, the insulin receptor (InR) pathway is involved in regulating key physiological processes, including juvenile hormone (JH) synthesis, vitellogenin production, and oocyte growth. This raises the question about which ligand (or ligands) binds to InR to trigger the above effects. We have cloned seven insulin-like peptides (BgILP1 to 7) from female Blattella germanica cockroaches and found that the brain expresses BgILP1 to 6, the fat body BgILP7, and the ovary BgILP2. Starvation induces the reduction of BgILP3, 5, and 6 mRNA levels in the brain, and the various BgILPs are differentially expressed during the gonadotrophic cycle. In addition, by knocking down the BgILPs we were able to identify compensatory regulation at transcriptional level between the different BgILPs, although none of the BgILP knockdown assays, including the knockdown of the seven BgILPs, produced the same phenotypes that we achieved by depleting InR. Taken together, the results indicate that B. germanica ILPs are differentially expressed in tissues and in response to physiological conditions, and that they are affected by compensatory regulation. This work was supported by grants BFU2010-15906 (Spanish Ministry of Science and Innovation (MICINN)), CGL2016-76011-R (Spanish Ministry of Economy and Competitiveness (MINECO) and FEDER) and 2014-SGR-619 (Secretaria d’Universitats i Recerca, Catalan Goverment) to J.L.M. |
| Databáze: | OpenAIRE |
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