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OBJECTIVES: The objective of this study was to determine the protective effects of antioxidants against oxidative damage in PC12 cells when treated with beta-amyloid (BA), a common source of reactive oxygen species (ROS) in older adult brains. METHODS: PC12 cells (2 × 10(5) cells/mL) were treated with secoisolariciresinol diglucoside (SDG, 50 or 100 μM) or control antioxidants, superoxide dismutase (SOD, 100 U/mL) or catalase (100 U/mL), for 2 hours, then treated with 10 μM BA for 24 hours. After antioxidant and BA treatments, ROS and lactate dehydrogenase (LDH) were measured using assay kits. Differences in ROS and LDH between control (no antioxidant and BA treatments) and experimental groups were analyzed by Student's t-tests. RESULTS: Cells treated with BA without an antioxidant treatment showed a significant reduction in LDH (p |
