In Vitro Bioaccessibility of Bioactive Compounds from Citrus Pomaces and Orange Pomace Biscuits

Autor: María Dolores del Castillo, Eduardo Dellacassa, Adriana Gámbaro, Tiziana Nardin, Roberto Larcher, Alejandra Medrano-Fernandez, Adriana Maite Fernández-Fernández
Přispěvatelé: Consejo Superior de Investigaciones Científicas (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Nacional de Investigación e Innovación (Uruguay), Universidad de la República (Uruguay), Pedeciba (Uruguay), Programa de Desarrollo de las Ciencias Básicas (Uruguay)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Citrus
antioxidant
Anti-Inflammatory Agents
Pharmaceutical Science
Bioaccessibility
Orange (colour)
01 natural sciences
Nobiletin
Antioxidants
Analytical Chemistry
chemistry.chemical_compound
Hesperidin
Mice
QD241-441
Tandem Mass Spectrometry
Drug Discovery
Food science
Citrus pomaces
Chromatography
High Pressure Liquid
anti-inflammatory
Neohesperidin
ABTS
Narirutin
diabetes
Diabetes
04 agricultural and veterinary sciences
Sensory analysis
040401 food science
bioaccessibility
Chemistry (miscellaneous)
Molecular Medicine
Digestion
citrus pomaces
α-glucosidase
Antioxidant
Citrus sinensis
Article
Cell Line
sensory analysis
Anti-inflammatory activity
0404 agricultural biotechnology
Functional biscuits
Animals
Humans
Physical and Theoretical Chemistry
Settore CHIM/10 - CHIMICA DEGLI ALIMENTI
Naringin
Flavonoids
Plant Extracts
010401 analytical chemistry
Organic Chemistry
functional biscuits
Pomace
0104 chemical sciences
Gastrointestinal Tract
Oxidative Stress
α-amylase
RAW 264.7 Cells
chemistry
Fruit
Zdroj: Molecules
Volume 26
Issue 12
Digital.CSIC. Repositorio Institucional del CSIC
instname
Molecules, Vol 26, Iss 3480, p 3480 (2021)
ISSN: 1420-3049
DOI: 10.3390/molecules26123480
Popis: This article belongs to the Special Issue Phytochemicals from Fruit and Vegetable By-Products and Wastes and Their Re-use.
The present investigation aimed to provide novel information on the chemical composition and in vitro bioaccessibility of bioactive compounds from raw citrus pomaces (mandarin varieties Clemenule and Ortanique and orange varieties Navel and Valencia). The effects of the baking process on their bioaccessibility was also assessed. Samples of pomaces and biscuits containing them as an ingredient were digested, mimicking the human enzymatic oral gastrointestinal digestion process, and the composition of the digests were analyzed. UHPLC-MS/MS results of the citrus pomaces flavonoid composition showed nobiletin, hesperidin/neohesperidin, tangeretin, heptamethoxyflavone, tetramethylscutellarein, and naringin/narirutin. The analysis of the digests indicated the bioaccessibility of compounds possessing antioxidant [6.6–11.0 mg GAE/g digest, 65.5–97.1 µmol Trolox Equivalents (TE)/g digest, and 135.5–214.8 µmol TE/g digest for total phenol content (TPC), ABTS, and ORAC-FL methods, respectively; significant reduction (p < 0.05) in Reactive Oxygen Species (ROS) formation under tert-butyl hydroperoxide (1 mM)-induced conditions in IEC-6 and CCD-18Co cells when pre-treated with concentrations 5–25 µg/mL of the digests], anti-inflammatory [significant reduction (p < 0.05) in nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages], and antidiabetic (IC50 3.97–11.42 mg/mL and 58.04–105.68 mg/mL for α-glucosidase and α-amylase inhibition capacities) properties in the citrus pomaces under study. In addition, orange pomace biscuits with the nutrition claims “no-added sugars” and “source of fiber”, as well as those with good sensory quality (6.9–6.7, scale 1–9) and potential health promoting properties, were obtained. In conclusion, the results supported the feasibility of citrus pomace as a natural sustainable source of health-promoting compounds such as flavonoids. Unfractionated orange pomace may be employed as a functional food ingredient for reducing the risk of pathophysiological processes linked to oxidative stress, inflammation, and carbohydrate metabolism, such as diabetes, among others.
This research was funded by ANII (grant POS_NAC_2016_1_130292), program EMHE-CSIC (grant MHE-200003), PEDECIBA-UdelaR, CSIC (project 201970E117) and Ministerio de Ciencia e Innovación (project PID2019-111510RB-I00).
Databáze: OpenAIRE
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