Effect of oral colchicine on T cell subsets, monocytes and concanavalin A-induced suppressor cell function in asthmatic patients
| Autor: | O Kuperman, Moshe Garty, M. Pecht, David Ilfeld, L. Netzer, A. Mazar, S. Spitzer, N. Trainin, G. Fink |
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| Rok vydání: | 1986 |
| Předmět: |
Adult
Male medicine.medical_specialty Cellular immunity T-Lymphocytes T cell Immunology T-Lymphocytes Regulatory Peripheral blood mononuclear cell Monocytes chemistry.chemical_compound Internal medicine Concanavalin A Cyclic AMP Humans Immunology and Allergy Medicine Colchicine Cytotoxic T cell Phytohaemagglutinin biology business.industry Monocyte Middle Aged Asthma Endocrinology medicine.anatomical_structure chemistry biology.protein Female business |
| Zdroj: | Clinical & Experimental Allergy. 16:407-416 |
| ISSN: | 0954-7894 |
| Popis: | Asthmatic patients have a deficiency of concanavalin A-(Con A) induced suppressor cell function. We tested whether oral colchicine 0.5 mg twice daily for 7 days could correct this immunoregulatory abnormality. Peripheral blood mononuclear cells were incubated with Con A and then suppression of proliferation was measured by coculture of these cells with healthy volunteers' mononuclear cells and phytohaemagglutinin. Sixteen asthmatic patients had significantly (P less than 0.002) decreased Con A-induced suppressor cell function (17.0 +/- 17.2%, mean +/- s.d.) as compared to 13 healthy volunteers (37.9 +/- 14.9%). Oral colchicine significantly (P less than 0.05) increased, though only partially corrected, these 16 asthmatic patients' Con A-induced suppressor cell function (28.1 +/- 14.3%). Asthmatic patients had an increased number of monocytes (691 +/- 289 vs 388 +/- 271/mm3 for normals, P less than 0.01) and a normal number of lymphocytes, Leu 4+ total T cells, Leu 3+ helper/inducer T cells, and Leu 2+ suppressor/cytotoxic T cells as well as a normal Leu 3/Leu 2 ratio. Oral colchicine significantly (P less than 0.005) decreased the number of monocytes (451 +/- 255/mm3) without significantly affecting the number of lymphocytes, Leu 4+, Leu 3+, or Leu 2+ T cells, or the Leu 3/Leu 2 ratio. These results are consistent with the hypothesis that the deficiency of Con A-induced suppressor cell function in asthmatic patients may be due, in part, to an increased number and/or abnormal activity of monocytes. If so, then oral colchicine may have partially corrected the deficiency of Con A-induced suppressor cell function by decreasing the number and/or modulating the activity of monocytes. |
| Databáze: | OpenAIRE |
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