Establishment of a green fluorescent protein tracing murine model focused on the functions of host components in necrosis repair and the niche of subcutaneously implanted glioma
| Autor: | Ke Lv, Jun Dong, Jingyun Jia, Aidong Wang, Quan-bin Zhang, Qing Lan, Jinshi Zhang, Qiang Huang, Lin-Ming He, Yanming Chen, Xingliang Dai, Xiaoyu Ma, Zhaohui Lu, Bin Liu, Chungang Dai |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Green Fluorescent Proteins Transplantation Heterologous Antigens Differentiation Myelomonocytic Mice Nude Mice Transgenic Biology Green fluorescent protein Mice Necrosis Nude mouse Antigens CD Cell Line Tumor Glioma Tumor Microenvironment medicine Animals Humans Progenitor cell Tumor microenvironment CD11b Antigen Neovascularization Pathologic Brain Neoplasms Tumor Necrosis Factor-alpha General Medicine Cell cycle Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease biology.organism_classification Mice Inbred C57BL Disease Models Animal Ki-67 Antigen Oncology Tumor necrosis factor alpha Stem cell Neoplasm Transplantation |
| Zdroj: | Oncology Reports. 31:657-664 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2013.2873 |
| Popis: | Due to progress in the research of glioma stem cells and the glioma niche, development of an animal model that facilitates the elucidation of the roles of the host tissue and cells is necessary. The aim of the present study was to develop a subcutaneous xenograft green fluorescent protein nude mouse model and use this model to analyze the roles of host cells in tumor necrosis repair. Tumors derived from the human glioma stem/progenitor cell line SU3 were subcutaneously implanted in green fluorescent protein nude mice. The implanted tumors were then passed from animal to animal for 10 generations. Finally, subcutaneous xenografts were assayed with traditional pathology, immunopathological techniques and fluorescence photography. For each generation, the tumorigenicity rate was 100%. Subcutaneous xenografts were rich in blood vessels, and necrotic and hemorrhagic foci, which highly expressed hypoxia-inducible factor-1α, tumor necrosis factor, Ki-67, CD68 and CD11b. In the interstitial tissue, particularly in old hemorrhagic foci, there were numerous cells expressing green fluorescent protein, CD68 and CD11b. Green fluorescent protein nude mouse subcutaneous xenografts not only consistently maintained the high invasiveness and tumorigenicity of glioma stem/progenitor cells, but also consisted of a high concentration of tumor blood vessels and necrotic and hemorrhagic foci. Subcutaneous xenografts also expressed high levels of tumor microenvironment-related proteins and host-derived tumor interstitial molecules. The model has significant potential for further research on tumor tissue remodeling and the tumor microenvironment. |
| Databáze: | OpenAIRE |
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