Stromal Cells Provide Signals Different from Cytokines for STAT5 Activation in Hematopoietic Cells
| Autor: | Nobuaki Yanai, Kazuhiro Mori, Masuo Obinata, Asako K. Sato, Tadashi Okubo |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Stromal cell
Physiology Cellular differentiation CD34 Gene Expression Cell Line Proto-Oncogene Proteins STAT5 Transcription Factor Lymph node stromal cell medicine Phosphorylation Molecular Biology bcl-2-Associated X Protein Chemistry Interleukin-7 Mesenchymal stem cell Hematopoietic stem cell Cell Biology General Medicine Hematopoietic Stem Cells Milk Proteins Cell biology DNA-Binding Proteins medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Trans-Activators Cancer research Interleukin-3 Stromal Cells Stem cell Cell Division Signal Transduction Adult stem cell |
| Zdroj: | Cell Structure and Function. 26:95-101 |
| ISSN: | 1347-3700 0386-7196 |
| DOI: | 10.1247/csf.26.95 |
| Popis: | After detachment from the stromal cells, hematopoietic stem cells are thought to differentiate to the cytokine-dependent stages where their growth and differentiation are promoted by these cytokines. To examine the stromal regulation of hematopoietic stem cells, we previously established a primitive hematopoietic stem-like cell line, THS119, whose growth was dependent on the bone marrow stromal cell line, TBR59, and from which IL-3- (THS119/IL-3) or IL-7- (THS119/IL-7) dependent cell lines were then generated. Using these cell lines, we examined the difference in signals mediated by the stromal cells and cytokines. The cytokine-dependent cell lines (THS119/IL-3 and THS119/IL-7) showed induction of STAT5 phosphorylation and target genes for STAT5 such as CIS, pim-1, p21 and bcl-xL upon addition of IL-3 or IL-7. IL-3 or IL-7 also induced STAT5 phosphorylation and STAT5 target genes of the stromal cell-dependent cell line, THS119, in the absence of stromal cells at levels similar to the cytokine-dependent cell lines. However, quite interestingly, TBR59 stromal cells could not induce STAT5 phosphorylation of THS119 cells, although they did induce STAT5 target genes in THS119 cells. In addition, the mRNAs for STAT5 target genes in THS119 cells on the stromal cells seemed to be more stable than those in the cytokine-dependent cell lines. Expression of the antiapoptotic genes bcl-2 and bcl-xL was higher in the stromal cell-dependent cell line than in the cytokine-dependent cell lines. These results suggested that stromal cells and cytokines may provide different signals for growth and differentiation of the hematopoietic cells. |
| Databáze: | OpenAIRE |
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