Revised locations of the hisI and pru (proline utilization) genes on the Pseudomonas aeruginosa chromosome map
| Autor: | Judith M. Carrigan, Robert Crockett, Dieter Haas, B. W. Holloway, Leda Soldati, Thomas Leisinger |
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| Rok vydání: | 1984 |
| Předmět: |
Genetics
Genotype Proline Genetic Linkage Pseudomonas aeruginosa Chromosome Mapping Chromosomes Bacterial Biology medicine.disease_cause Transduction (genetics) Plasmid Genes Bacterial Transduction Genetic Chromosome regions Gene cluster medicine Histidine Molecular Biology Gene Crosses Genetic Recombination Plasmids |
| Zdroj: | Molecular and General Genetics MGG. 193:431-436 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00382080 |
| Popis: | The location of genes in the vicinity of the major FP2 origin on the chromosome of Pseudomonas aeruginosa PAO has been revised. The markers hisI (a transduction group of histidine biosynthetic genes) and pru (a gene cluster encoding proline utilization functions) were located in the 90 to 95/0 min chromosome region by a series of plate matings mediated by R68.45. Three-factor-crosses using this plasmid established the following marker order: pur-67 pru hisI/cys-59 proB ilvB/C. Genetic evidence is presented to confirm the previous observations that FP2 can mobilize the chromosome from at least two origins near proB and in both directions. Thus, when markers in this chromosome region are analyzed by FP2 crosses only, the mapping data may be difficult to interpret. This complication can be overcome by the use of R68.45 and Tfr (transposon-facilitated recombination) or Hfr donors. |
| Databáze: | OpenAIRE |
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