Structure of the capsular polysaccharide and the O-side- chain of the lipopolysaccharide from Acetabacter methanolicus MB 584 (IMET (10945), and of oligosaccharides resulting from their degradation by the bacteriophage Acml
ISSN: | 0008-6215 |
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DOI: | 10.1016/0008-6215(91)80014-e |
Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bcb7b0f8a85ac5e35ae7aae2089ea50 https://doi.org/10.1016/0008-6215(91)80014-e |
Rights: | CLOSED |
Přírůstkové číslo: | edsair.doi.dedup.....3bcb7b0f8a85ac5e35ae7aae2089ea50 |
Autor: | H D, Grimmecke, U, Mamat, W, Lauk, A S, Shashkov, Y A, Knirel, E V, Vinogradov, N K, Kochetkov |
Rok vydání: | 1991 |
Předmět: |
Lipopolysaccharides
Molecular Sequence Data Disaccharide Oligosaccharides Polysaccharide Biochemistry Analytical Chemistry chemistry.chemical_compound Hydrolase Acetobacter Bacteriophages Bacterial Capsules Glucan chemistry.chemical_classification biology Strain (chemistry) Polysaccharides Bacterial Organic Chemistry General Medicine biology.organism_classification carbohydrates (lipids) Carbohydrate Sequence chemistry Galactose Carbohydrate conformation |
Zdroj: | Carbohydrate Research. 220:165-172 |
ISSN: | 0008-6215 |
DOI: | 10.1016/0008-6215(91)80014-e |
Popis: | Acetobacter methanolicus MB 70 was shown to be related to the type strain of this species MB 58/4 (IMET 10945) having the same galactan-->2)-beta-D-Gal f-(1-->3)-beta-D-Gal p-(1-->as the capsular polysaccharide (CPS) and the O-side-chain of the lipopolysaccharide (LPS). Additionally, a glucan built up of the disaccharide repeating unit-->6)-alpha-D-Glc p-(1-->2)-alpha-D-Glc p-(1-->was identified in strain MB 70. In the CPS, the polymers were present in the ratio approximately 1:1, whereas the glucan preponderated in the LPS. Bacteriophage Acm6 specific to A. methanolicus MB 70 hydrolysed selectively the glucan component of both CPS and LPS. Structural elucidation of the resulting oligosaccharides led to the identification of the phage-associated depolymerase as an endo-alpha-(1-->6)-D-glucopyranoside hydrolase. |
Databáze: | OpenAIRE |
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