Multifunctional CD4(+) T cells correlate with active Mycobacterium tuberculosis infection

Autor: Giuseppe Gelsomino, Alfredo Salerno, Simone A. Joosten, Alessandro Sanduzzi, Domenico Galati, Francesco Dieli, Giuliana Guggino, Alessandro Matarese, Willeke P. J. Franken, Tom H. M. Ottenhoff, Paola Di Carlo, Lucina Titone, Nadia Caccamo, Marialuisa Bocchino
Přispěvatelé: Caccamo N, Guggino G, Joosten SA, Gelsomino G, Di Carlo P, Titone Lanza di Scalea L, Galati D, Bocchino M, Matarese A, Salerno A, Sanduzzi A, Franken WP, Ottenhoff TH, Dieli F, Caccamo, N., Guggino, G., Joosten, S. A., Gelsomino, G., Di Carlo, P., Titone, L., Galati, D., Bocchino, Marialuisa, Matarese, A., Salerno, A., SANDUZZI ZAMPARELLI, Alessandro, Franken, W. P., Ottenhoff, T. H., Dieli, F.
Rok vydání: 2010
Předmět:
Interleukin 2
Adult
CD4-Positive T-Lymphocytes
Male
Tuberculosis
Settore MED/17 - Malattie Infettive
Immunology
Cell Separation
Biology
Lymphocyte Activation
Flow cytometry
Mycobacterium tuberculosis
03 medical and health sciences
0302 clinical medicine
Immune system
Bacterial Proteins
CD4(+) T cells Cytokines Mycobacterium tuberculosis infection Tuberculosis disease interferon-gamma immunological memory disease responses protection cytokine immunity bcg vaccination virus
medicine
Immunology and Allergy
Distribution (pharmacology)
Humans
Cytokine
Tuberculosis
Pulmonary
030304 developmental biology
Tuberculosis disease
Settore MED/04 - Patologia Generale
0303 health sciences
Antigens
Bacterial
medicine.diagnostic_test
Mycobacterium tuberculosis infection
Middle Aged
biology.organism_classification
medicine.disease
Flow Cytometry
Phenotype
Virology
CD4+ T cells
Bacterial Load
3. Good health
Acute Disease
Chronic Disease
Cytokines
Tumor necrosis factor alpha
Acyltransferases
030215 immunology
medicine.drug
Zdroj: European Journal of Immunology; Vol 40
European Journal of Immunology
European Journal of Immunology, 40(8), 2211-2220
ISSN: 1521-4141
Popis: Th1 CD4(+) T cells and their derived cytokines are crucial for protection against Mycobacterium tuberculosis. Using multiparametic flow cytometry, we have evaluated the distribution of seven distinct functional states (IFN-gamma/IL-2/TNF-alpha triple expressors, IFN-gamma/IL-2, IFN-gamma/TNF-alpha or TNF-alpha/IL-2 double expressors or IFN-gamma, IL-2 or TNF-alpha single expressors) of CD4(+) T cells in individuals with latent M. tuberculosis infection (LTBI) and active tuberculosis (TB). We found that triple expressors, while detectable in 85-90%TB patients, were only present in 10-15% of LTBI subjects. On the contrary, LTBI subjects had significantly higher (12- to 15-fold) proportions of IL-2/IFN-gamma double and IFN-gamma single expressors as compared with the other CD4(+) T-cell subsets. Proportions of the other double or single CD4(+) T-cell expressors did not differ between TB and LTBI subjects. These distinct IFN-gamma, IL-2 and TNF-alpha profiles of M. tuberculosis-specific CD4(+) T cells seem to be associated with live bacterial loads, as indicated by the decrease in frequency of multifunctional T cells in TB-infected patients after completion of anti-mycobacterial therapy. Our results suggest that phenotypic and functional signatures of CD4(+) T cells may serve as immunological correlates of protection and curative host responses, and be a useful tool to monitor the efficacy of anti-mycobacterial therapy.
Databáze: OpenAIRE
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