Modification of bacterial nanocellulose properties through mutation of motility related genes in Komagataeibacter hansenii ATCC 53582
Autor: | Małgorzata Ryngajłło, Katarzyna Kubiak, Przemysław Rytczak, Piotr Paluch, Stanisław Bielecki, Paulina Jacek |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
Movement Mutant Motility Bioengineering medicine.disease_cause 01 natural sciences Homology (biology) Nanocellulose 03 medical and health sciences Bacterial Proteins 010608 biotechnology Spectroscopy Fourier Transform Infrared medicine Carbon-13 Magnetic Resonance Spectroscopy Cellulose Molecular Biology Gene 030304 developmental biology 0303 health sciences Mutation Sequence Homology Amino Acid Chemistry General Medicine Phenotype Complementation Biochemistry Genes Bacterial Acetobacteraceae Nanoparticles Biotechnology |
Zdroj: | New Biotechnology. 52:60-68 |
ISSN: | 1871-6784 |
Popis: | Bacterial nanocellulose (BNC) produced by Komagataeibacter hansenii has received significant attention due to its unique supernetwork structure and properties. It is nevertheless necessary to modify bacterial nanocellulose to achieve materials with desired properties and thus with broader areas of application. The aim here was to influence the 3D structure of BNC by genetic modification of the cellulose producing K. hansenii strain ATCC 53582. Two genes encoding proteins with homology to the MotA and MotB proteins, which participate in motility and energy transfer, were selected for our studies. A disruption mutant of one or both genes and their respective complementation mutants were created. The phenotype analysis of the disruption mutants showed a reduction in motility, which resulted in higher compaction of nanocellulose fibers and improvement in their mechanical properties. The data strongly suggest that these genes play an important role in the formation of BNC membrane by Komagataeibacter species. |
Databáze: | OpenAIRE |
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