Macrodomain organization of the Escherichia coli chromosome
| Autor: | Michèle Valens, François Cornet, Frédéric Boccard, Stéphanie Penaud, Michèle Rossignol |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Genetics
DNA Bacterial Recombination Genetic General Immunology and Microbiology Models Genetic General Neuroscience Circular bacterial chromosome Chromosome Biology Chromosomes Bacterial medicine.disease_cause General Biochemistry Genetics and Molecular Biology Article Chromosome segregation chemistry.chemical_compound chemistry medicine Escherichia coli Sister chromatids Nucleic Acid Conformation Site-specific recombination Molecular Biology Recombination DNA |
| Popis: | We have explored the Escherichia coli chromosome architecture by genetic dissection, using a site-specific recombination system that reveals the spatial proximity of distant DNA sites and records interactions. By analysing the percentages of recombination between pairs of sites scattered over the chromosome, we observed that DNA interactions were restricted to within subregions of the chromosome. The results indicated an organization into a ring composed of four macrodomains and two less-structured regions. Two of the macrodomains defined by recombination efficiency are similar to the Ter and Ori macrodomains observed by FISH. Two newly characterized macrodomains flank the Ter macrodomain and two less-structured regions flank the Ori macrodomain. Also the interactions between sister chromatids are rare, suggesting that chromosome segregation quickly follows replication. These results reveal structural features that may be important for chromosome dynamics during the cell cycle. |
| Databáze: | OpenAIRE |
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